Scientific Publications
Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.
Wang, Dongxu; Ren, Jing; Li, Jiping; Li, Xiuying; Ying, Jinchi; Jiang, Tiantian; Wang, Zhen; Pan, Zheng; Guo, Qianqian; Li, Chunyi; Zhang, Guokun
M8 Microscope and Scanner Utilized to Explore Deer Antler Stem Cell Conditioned Media\'s Potential in Alleviating Type 1 Diabetes via NF-κB Pathway Inhibition
A study, using the M8 microscope and slide scanner, delved into the impact of Type 1 diabetes mellitus (T1D) on human health. Type 1 diabetes mellitus (T1D) poses a significant threat to human health, primarily due to absolute insulin deficiency, leading to elevated blood glucose levels and potentially life-threatening complications such as liver injury. Mesenchymal stem cell (MSC) transplantation has emerged as a promising treatment for T1D and its associated liver injuries, leveraging the regenerative and immunomodulatory properties of MSCs. The study explored the therapeutic potential of conditioned medium from deer antler stem cells (AnSC-CM) in T1D and diabetic liver injuries. Results indicate that AnSC-CM not only alleviates T1D symptoms but also mitigates T1D-induced liver injury, outperforming bone marrow MSC-conditioned medium (BMSC-CM). Mechanistic insights suggest that the therapeutic effects of AnSC-CM may be mediated through targeting the NF-κB signaling pathway. The research proposes a novel strategy utilizing alternative stem cell conditioned mediums for effective treatment of T1D and associated liver injuries in clinical settings.
Result: Research findings demonstrated that AnSC-CM effectively relieved symptoms associated with T1D, including decreased body weight, elevated blood glucose levels, islet lesions, and diminished insulin secretion. Additionally, AnSC-CM treatment exhibited notable improvements in liver function and the mitigation of T1D-induced liver injury in mice. The therapeutic efficacy of AnSC-CM surpassed that of BMSC-CM. Analysis of underlying mechanisms unveiled significant downregulation of the NF-κB signaling pathway in both pancreatic and liver tissues by AnSC-CM. These results suggest that AnSC-CM\'s therapeutic effects on T1D and associated liver injury induced by STZ may be attributed to its modulation of the NF-κB signaling pathway.
@article{nokey,
title = {Conditioned Media from Deer Antler Stem Cells Effectively Alleviate Type 1 Diabetes Mellitus Possibly via Inhibiting the NF-κB Signaling Pathway},
author = {Dongxu Wang and Jing Ren and Jiping Li and Xiuying Li and Jinchi Ying and Tiantian Jiang and Zhen Wang and Zheng Pan and Qianqian Guo and Chunyi Li and Guokun Zhang},
url = {https://www.imrpress.com/journal/FBL/29/3/10.31083/j.fbl2903096/htm},
year = {2024},
date = {2024-03-11},
journal = {Front. Biosci. (Landmark Ed)},
volume = {29},
issue = {3},
abstract = {Background: Type 1 diabetes mellitus (T1D) represents a severe threat to human health. Persistent hyperglycemia and dyslipidemia can lead to damaged liver function, while effective interventions for these complications are currently lacking. Deer antler stem cells (AnSCs), a novel type of adult stem cells, significantly reduced liver injury, which was speculated to be achieved through the paracrine pathway. Methods: In this study, AnSC-conditioned medium (AnSC-CM) was used to treat C57BL/6 mice with T1D symptoms induced by streptozotocin (STZ). The therapeutic effects of AnSC-CM on T1D were evaluated, and the underlying mechanism was investigated. Results: It was shown that AnSC-CM alleviated the T1D symptom: decreased body weight, increased blood glucose levels and islet lesions, and reduced insulin secretion. Moreover, AnSC-CM treatment improved liver function and mitigated liver injury in T1D mice. Impressively, the therapeutic effects of AnSC-CM on T1D were better than those of bone marrow mesenchymal stem cell-CM (BMSC-CM). The mechanistic study revealed that AnSC-CM significantly downregulated the NF-κB signaling pathway in both pancreatic and liver tissues. Conclusions: Therapeutic effects of AnSC-CM on STZ-induced T1D and liver injury may be achieved through targeting the NF-κB signaling pathway.},
keywords = {M8},
pubstate = {published},
tppubtype = {article}
}
Hansen,; E.,; Wang,; M.,; Rolling,; C.,; & Holaska,; M., J.
PreciPoint Slide Scanning Microscope Unveils the Invasive Phenotype of MCF7 Cells under Emerin Deficiency
Using PreciPoint’s slide scanning microscope, researchers investigated how cancer cells migrate through blood vessels during metastasis. They observed that cancer cells adapt to pass through narrow gaps in the endothelium by altering their nuclei, which become more deformable. The study focused on the role of emerin protein in this process. Researchers found that invasive breast cancer cells, which have lower levels of emerin, exhibit smaller and misshapen nuclei compared to non-cancerous cells. This reduction in emerin was associated with higher rates of metastasis. To validate their hypothesis, the researchers manipulated emerin levels in noninvasive MCF7 cells and observed similar changes in nucleus size and shape, leading to impaired cell migration. This effect was consistent when emerin levels were reduced in invasive breast cancer cells. Furthermore, the analysis of breast cancer patient samples revealed a negative correlation between emerin expression and cancer invasiveness, suggesting its potential as a biomarker for tumor progression. The findings indicate that emerin loss plays a crucial role in promoting invasive transformation, highlighting its significance in cancer metastasis.
Result: To investigate how reducing emerin levels affects MCF7 cells, researchers created various MCF7 cell lines. These cell lines were engineered to produce one of three distinct emerin shRNA sequences labeled as A, B, or C, alongside a control group that expressed a scrambled shRNA sequence obtained from Genecopoeia. Immunohistochemistry and tissue microarray analyses were conducted on these cell lines. For the tissue microarray analysis, tissue microarrays underwent deparaffinization and rehydration procedures. Following additional tissue treatments, images were captured utilizing the Precipoint slide scanning microscope.
@article{nokey,
title = {Emerin deficiency drives MCF7 cells to an invasive phenotype},
author = {Hansen and E. and Wang and M. and Rolling and C. and & Holaska and J. M.},
url = {https://www.biorxiv.org/content/10.1101/2024.02.21.581379v2.full.pdf
https://www.biorxiv.org/content/10.1101/2024.02.21.581379v2},
doi = {10.1101/2024.02.21.581379},
year = {2024},
date = {2024-02-24},
journal = {Cold Spring Harbor Laboratory},
abstract = {During metastasis, cancer cells traverse the vasculature by squeezing through very small gaps in the endothelium. Thus, nuclei in metastatic cancer cells must become more malleable to move through these gaps. Our lab showed invasive breast cancer cells have 50% less emerin protein resulting in smaller, misshapen nuclei, and higher metastasis rates than non-cancerous controls. Thus, emerin deficiency was predicted to cause increased nuclear compliance, cell migration, and metastasis. We tested this hypothesis by downregulating emerin in noninvasive MCF7 cells and found emerin knockdown causes smaller, dysmorphic nuclei, resulting in increased impeded cell migration. Emerin reduction in invasive breast cancer cells showed similar results. Supporting the clinical relevance of emerin reduction in cancer progression, our analysis of 192 breast cancer patient samples showed emerin expression inversely correlates with cancer invasiveness. We conclude emerin loss is an important driver of invasive transformation and has utility as a biomarker for tumor progression.
},
keywords = {Fritz, M8},
pubstate = {published},
tppubtype = {article}
}
Yu, Jing; Gao, Boyuan; Li, Danning; Li, Shuang; Chiang, Vincent L.; Li, Wei; Zhou, Chenguang
M8 Microscope Reveals PtrLBD39 Overexpression Hinders Primary and Secondary Growth in Populus Trichocarpa
Researchers used M8 microscope and scanner to investigate the mechanisms underlying primary and secondary growth in trees, crucial for height and stem diameter increments, respectively, which are essential for woody biomass production. They discovered PtrLBD39, a transcription factor highly specific to stem phloem in Populus trichocarpa. Through ectopic expression of PtrLBD39 in P. trichocarpa, researchers observed a significant retardation in both primary and secondary growth. Their comprehensive analysis, including RNA-seq, ChIP-seq, and weighted gene co-expression network analysis (WGCNA), unveiled PtrLBD39\'s role in regulating transcription factors associated with vascular tissue development, wood formation, hormonal signaling, and enzymes responsible for wood components. This regulatory function resulted in growth inhibition, reduced fibrocyte secondary cell wall thickness, and diminished wood production. The study suggests that PtrLBD39 acts as a repressor, affecting both primary and secondary growth when ectopically expressed in P. trichocarpa.
Result: The stem segments, after treatment, underwent cutting into 10-micrometer sections and staining with solutions containing 5% Safranin O, 1.25% Fast Green, and 0.1% Toluidine Blue. Subsequently, micrographs of the stem sections were obtained using an M8 digital microscope and scanner. The findings of the study suggest that PtrLBD39 functions as a suppressor, impacting both primary and secondary growth upon its ectopic expression in P. trichocarpa.
@article{nokey_27,
title = {Ectopic Expression of PtrLBD39 Retarded Primary and Secondary Growth in Populus trichocarpa},
author = {Jing Yu and Boyuan Gao and Danning Li and Shuang Li and Vincent L. Chiang and Wei Li and Chenguang Zhou},
url = {https://doi.org/10.3390/ijms25042205},
doi = {10.3390/ijms25042205},
year = {2024},
date = {2024-02-12},
urldate = {2024-02-12},
journal = {International Journal of Molecular Sciences},
volume = {25},
issue = {4},
abstract = {Primary and secondary growth of trees are needed for increments in plant height and stem diameter, respectively, affecting the production of woody biomass for applications in timber, pulp/paper, and related biomaterials. These two types of growth are believed to be both regulated by distinct transcription factor (TF)-mediated regulatory pathways. Notably, we identified PtrLBD39, a highly stem phloem-specific TF in Populus trichocarpa and found that the ectopic expression of PtrLBD39 in P. trichocarpa markedly retarded both primary and secondary growth. In these overexpressing plants, the RNA-seq, ChIP-seq, and weighted gene co-expression network analysis (WGCNA) revealed that PtrLBD39 directly or indirectly regulates TFs governing vascular tissue development, wood formation, hormonal signaling pathways, and enzymes responsible for wood components. This regulation led to growth inhibition, decreased fibrocyte secondary cell wall thickness, and reduced wood production. Therefore, our study indicates that, following ectopic expression in P. trichocarpa, PtrLBD39 functions as a repressor influencing both primary and secondary growth.
},
keywords = {M8},
pubstate = {published},
tppubtype = {article}
}
Rusche,; D.,; Englert,; N.,; Runz,; M.,; Hetjens,; S.,; Langner,; C.,; Gaiser,; T.,; & Weis,; C.-A.,
M8 Microscope and Scanner Assists Researchers in Exploring the Challenges of Detecting Tumor Budding in Colorectal Carcinoma Histology
In colorectal carcinoma (CRC) research, the M8 microscope and scanner played a pivotal role in a study aimed at predicting post-surgery treatment requirements by discerning crucial tumor features within whole slide images of solid tumors. Addressing this challenge with a small CRC dataset, researchers explored two distinct approaches. Firstly, a conventional tile-level training method was examined, incorporating various data augmentation techniques to counter the memorization effect in a noisy label setting. Secondly, a multi-instance learning (MIL) approach at the case level was explored, adapting data augmentation to prevent over-fitting in the context of limited data. The tile-level strategy proved ineffective due to a scarcity of informative image tiles per case, while the MIL approach, coupled with post-feature vector creation data augmentation, successfully predicted nodal status based on expert-derived budding scores for these cases.
Result: The dataset consisted of 29 whole slide images (WSIs), including 21 for tumors, one each for high-grade and low-grade intra-epithelial neoplasia (IEN), five for ulcerative colitis, and one for healthy tissue. The entire tissue sections were scanned and saved in the .svs format for further analysis using the M8 microscope and scanner. The study demonstrated the effectiveness of the MIL method in identifying predictive factors such as tumor budding, even within the constraints of a limited dataset size by incorporating data augmentation techniques.
@article{nokey,
title = {Unraveling a Histopathological Needle-in-Haystack Problem: Exploring the Challenges of Detecting Tumor Budding in Colorectal Carcinoma Histology},
author = {Rusche and D. and Englert and N. and Runz and M. and Hetjens and S. and Langner and C. and Gaiser and T. and & Weis and C.-A.},
url = {https://www.mdpi.com/2076-3417/14/2/949/pdf?version=1706061826},
doi = {10.3390/app14020949},
year = {2024},
date = {2024-01-22},
journal = {Applied Sciences},
volume = {14},
issue = {2},
pages = {949},
abstract = {Background: In this study focusing on colorectal carcinoma (CRC), we address the imperative task of predicting post-surgery treatment needs by identifying crucial tumor features within whole slide images of solid tumors, analogous to locating a needle in a histological haystack. We evaluate two approaches to address this challenge using a small CRC dataset. Methods: First, we explore a conventional tile-level training approach, testing various data augmentation methods to mitigate the memorization effect in a noisy label setting. Second, we examine a multi-instance learning (MIL) approach at the case level, adapting data augmentation techniques to prevent over-fitting in the limited data set context. Results: The tile-level approach proves ineffective due to the limited number of informative image tiles per case. Conversely, the MIL approach demonstrates success for the small dataset when coupled with post-feature vector creation data augmentation techniques. In this setting, the MIL model accurately predicts nodal status corresponding to expert-based budding scores for these cases. Conclusions: This study incorporates data augmentation techniques into a MIL approach, highlighting the effectiveness of the MIL method in detecting predictive factors such as tumor budding, despite the constraints of a limited dataset size.
},
keywords = {M8},
pubstate = {published},
tppubtype = {article}
}
Stallmeyer,; B.,; Bühlmann,; C.,; Stakaitis,; R.,; Dicke,; A.-K.,; Ghieh,; F.,; Meier,; L.,; Zoch,; A.,; MacLeod,; M., D.; Steingröver,; J.,; Okutman,; Ö.,; Fietz,; D.,; Pilatz,; A.,; Escamilla,; R., A.; Xavier,; M.,; Ruckert,; C.,; Persio,; D., S.; Neuhaus,; N.,; Gurbuz,; S., A.; Şalvarci,; A.,; Tüttelmann, …; F.,
O8 Oil Microscope and Scanner Reveals Inherited Defects of piRNA Biogenesis Leading to Transposon De-Repression, Impaired Spermatogenesis, and Human Male Infertility
In an exploration of the genetic intricacies underlying piRNA dysfunction in humans, researchers leveraged the O8 oil digital microscope and slide scanner. The study delved into the critical functions of Piwi-interacting RNAs (piRNAs) in transposon silencing, germ cell maturation, and male fertility. Examining 39 infertile men with biallelic variants in 14 piRNA pathway genes, including novel candidates like PIWIL1 and GTSF1, the research uncovered distinct testicular phenotypes, ranging from complete germ cell loss to the production of abnormal spermatozoa. Notably, impaired piRNA biogenesis in spermatogonia led to transposon de-silencing, validated by elevated LINE1 expression. The study also revealed co-dependencies within the human piRNA pathway, as the abolished expression of both encoded proteins and additional piRNA factors contributed to spermatogenic failure. These findings establish the disrupted piRNA pathway as a significant cause of human infertility, providing crucial insights into transposon silencing in male germ cells.
Result: Testicular biopsies from individuals in the MERGE cohort and control subjects were acquired through testicular sperm extraction (TESE) procedures or histological assessment. These biopsies were fixed in Bouin’s solution overnight, followed by multiple processes. A the end, the processed slides were examined and documented using the O8 oil digital microscope and scanner. The study unveiled intricate co-dependencies within the human piRNA pathway. The study also demonstrated that the absence of expression in both the encoded proteins and additional piRNA factors played a significant role in contributing to spermatogenic failure.
@article{nokey,
title = {Inherited defects of piRNA biogenesis cause transposon de-repression, impaired spermatogenesis, and human male infertility},
author = {Stallmeyer and B. and Bühlmann and C. and Stakaitis and R. and Dicke and A.-K. and Ghieh and F. and Meier and L. and Zoch and A. and MacLeod and D. M. and Steingröver and J. and Okutman and Ö. and Fietz and D. and Pilatz and A. and Escamilla and A. R. and Xavier and M. and Ruckert and C. and Persio and S. D. and Neuhaus and N. and Gurbuz and A. S. and Şalvarci and A. and … Tüttelmann and F.},
url = {https://assets.researchsquare.com/files/rs-3710476/v1/14e3a3fa-f9d8-4572-9b1b-9857f92be1bf.pdf?c=1705956027
https://www.researchsquare.com/article/rs-3710476/v1},
doi = {10.21203/rs.3.rs-3710476/v1},
year = {2024},
date = {2024-01-09},
journal = {Research Square Platform LLC.},
abstract = {Piwi-interacting RNAs (piRNAs) are crucial for transposon silencing, germ cell maturation, and fertility in male mice. Here, we report on the genetic landscape of piRNA dysfunction in humans and present 39 infertile men carrying biallelic variants in 14 different piRNA pathway genes, including PIWIL1, GTSF1, GPAT2, MAEL, TDRD1, and DDX4 as novel disease genes. The testicular phenotypes repeatedly differ from those of the respective knockout mice and range from complete germ cell loss to the production of a few morphologically abnormal spermatozoa. LINE1 expression in spermatogonia links impaired piRNA biogenesis to transposon de-silencing and serves to classify variants as functionally relevant. Furthermore, an abolished expression of not only the encoded proteins but also of additional piRNA factors reveals co-dependencies within the human pathway. These results establish the disrupted piRNA pathway as a major cause of human spermatogenic failure and provide insights into transposon silencing in human male germ cells.
},
keywords = {O8},
pubstate = {published},
tppubtype = {article}
}
Shi,; H.,; Yuan,; X.,; Liu,; G.,; & Fan,; W.,
M8 Digital Microscope and Slide Scanning Used for Enhanced Histological Analysis of Wound Tissue
Utilizing the M8 microscope and scanner, this study harnessed the power of bioinformatics and machine learning to scrutinize microarray data sourced from the Gene Expression Omnibus (GEO) database, pinpointing crucial genes linked to Diabetic Foot Ulcers (DFU). The investigation employed GSE68183 and GSE80178 datasets as the training set, employing advanced techniques such as LASSO and SVM-RFE machine learning models through the glmnet and e1071 packages, respectively. Model validation ensued using both the training set and an independent validation set (GSE134431). Subsequent enrichment analyses, encompassing GSEA and GSVA, were applied to the identified genes. Immune functional and immune-related analyses were conducted to unravel the biological significance. The study culminated in a validation phase, employing immunohistochemistry (IHC) to corroborate the significance of the identified model genes.
Result: After preservation in 4% paraformaldehyde for 48 hours, the wound tissue underwent conventional paraffin embedding and sectioning procedures. Subsequently, staining procedures were employed, including hematoxylin and eosin, along with Masson’s trichrome stain, to enhance visualization of tissue characteristics. The stained tissues were meticulously scrutinized using a state-of-the-art M8 digital microscope and slide scanning device, ensuring precise and detailed examination of the histological features.
@article{nokey,
title = {Identifying and Validating GSTM5 as an Immunogenic Gene in Diabetic Foot Ulcer Using Bioinformatics and Machine Learning},
author = {Shi and H. and Yuan and X. and Liu and G. and & Fan and W.},
url = {https://www.tandfonline.com/doi/full/10.2147/JIR.S442388},
doi = {10.2147/JIR.S442388},
year = {2023},
date = {2023-12-19},
journal = {Journal of Inflammation Research},
volume = {16},
pages = {6241-6256},
abstract = {Background
A diabetic foot ulcer (DFU) is a serious, long-term condition associated with a significant risk of disability and mortality. However, research on its biomarkers is still limited. This study utilizes bioinformatics and machine learning methods to identify immune-related biomarkers for DFU and validates them through external datasets and animal experiments.
Methods
This study used bioinformatics and machine learning to analyze microarray data from the Gene Expression Omnibus (GEO) database to identify key genes associated with DFU. Animal experiments were conducted to validate these findings. This research employs the datasets GSE68183 and GSE80178 retrieved from the GEO database as the training dataset for building a gene machine learning model, and after conducting differential analysis on the data, this study used package glmnet and package e1071 to construct LASSO and SVM-RFE machine learning models, respectively. Subsequently, we validated the model using the training set and validation set (GSE134431). We conducted enrichment analysis, including GSEA and GSVA, on the model genes. We also performed immune functional analysis and immune-related analysis on the model genes. Finally, we conducted immunohistochemistry (IHC) validation on the model genes.
Results
This study identifies GSTM5 as a potential immune-related key target in DFU using machine learning and bioinformatics methods. Subsequent validation through external datasets and IHC experiments also confirms GSTM5 as a critical biomarker for DFU. The gene may be associated with T cells regulatory (Tregs) and T cells follicular helper, and it influences the NF-κB, GnRH, and MAPK signaling pathway.
Conclusion
This study identified and validated GSTM5 as a biomarker for DFU. This finding may potentially provide a target for immune therapy for DFU.},
keywords = {M8},
pubstate = {published},
tppubtype = {article}
}
A diabetic foot ulcer (DFU) is a serious, long-term condition associated with a significant risk of disability and mortality. However, research on its biomarkers is still limited. This study utilizes bioinformatics and machine learning methods to identify immune-related biomarkers for DFU and validates them through external datasets and animal experiments.
Methods
This study used bioinformatics and machine learning to analyze microarray data from the Gene Expression Omnibus (GEO) database to identify key genes associated with DFU. Animal experiments were conducted to validate these findings. This research employs the datasets GSE68183 and GSE80178 retrieved from the GEO database as the training dataset for building a gene machine learning model, and after conducting differential analysis on the data, this study used package glmnet and package e1071 to construct LASSO and SVM-RFE machine learning models, respectively. Subsequently, we validated the model using the training set and validation set (GSE134431). We conducted enrichment analysis, including GSEA and GSVA, on the model genes. We also performed immune functional analysis and immune-related analysis on the model genes. Finally, we conducted immunohistochemistry (IHC) validation on the model genes.
Results
This study identifies GSTM5 as a potential immune-related key target in DFU using machine learning and bioinformatics methods. Subsequent validation through external datasets and IHC experiments also confirms GSTM5 as a critical biomarker for DFU. The gene may be associated with T cells regulatory (Tregs) and T cells follicular helper, and it influences the NF-κB, GnRH, and MAPK signaling pathway.
Conclusion
This study identified and validated GSTM5 as a biomarker for DFU. This finding may potentially provide a target for immune therapy for DFU.
Drexler,; K.,; Zenderowski,; V.,; Schreieder,; L.,; Koschitzki,; K.,; Karrer,; S.,; Berneburg,; M.,; Haferkamp,; S.,; & Niebel,; D.,
PreciPoint M8 Microscope and Scanner Used to Study Melanoma Variances
Researchers used the M8 microscope, scanner, and ViewPoint Software to delve into the distinct connective tissue alterations within sun-exposed skin due to prolonged sun exposure. The escalation in sun exposure and resultant sunburns leads to heightened mole formation, alongside an increased risk of both melanomas and non-melanoma skin cancers. The inquiry into the disparity between chronic sun damage and intermittent exposure raises uncertainties about their specific influence on individual melanoma susceptibility. Investigating correlations between tissue modifications, melanoma subtypes, and the body sites exposed to varying sunlight conditions, findings reveal diverse tissue changes proximal to moles and melanomas, irrespective of patient age and tumor site. This discovery elucidates the intricate biological repercussions of sunlight on pigment cells, the precursors to moles and melanomas, and also underscores the imperative to discern nuances among melanoma subtypes for a comprehensive understanding of their etiology.
Result: The photomicrographs were taken after scanning the slides using a PreciPoint M8 microscope and scanner with the ViewPointLight software for imaging. The pivotal revelation of the study lies in the discernible contrast in the depth of actinic elastosis (AE) and tumor-associated elastosis grade (TEG) layers between superficial spreading melanoma (SSM) and nodular malignant melanoma (NMM). Specifically, the mean thickness of both AE and TEG was markedly lower in SSM compared to NMM. The study also affirmed a correlation between chronic solar damage (CSD) and lentigo maligna melanoma (LMM), distinguishing it from other clinical subtypes of melanomas.
@bachelorthesis{nokey,
title = {Subtypes of Melanomas Associated with Different Degrees of Actinic Elastosis in Conventional Histology, Irrespective of Age and Body Site, Suggesting Chronic Ultraviolet Light Exposure as Driver for Lentigo Maligna Melanoma and Nodular Melanoma},
author = {Drexler and K. and Zenderowski and V. and Schreieder and L. and Koschitzki and K. and Karrer and S. and Berneburg and M. and Haferkamp and S. and & Niebel and D.},
url = {https://www.mdpi.com/2072-6694/16/1/1},
doi = {10.3390/cancers16010001},
year = {2023},
date = {2023-12-19},
journal = {Cancers},
volume = {16},
issue = {1},
pages = {1},
abstract = {(1) Background: Ultraviolet (UV) radiation and sunburns are associated with an increased incidence of acquired nevi and melanomas. However, the data are controversial as to whether chronic UV exposure or high intermittent UV exposure is the major carcinogenic factor in melanocytic tumors. In this study, we compared the degree of actinic elastosis (AE) as a surrogate for lifetime UV exposure in nevi and different clinical melanoma subtypes (i.e., superficial spreading melanoma (SSM), nodular malignant melanoma (NMM), acral lentiginous melanoma (ALM), and lentigo maligna melanoma (LMM)) with respect to clinical variables (age, sex, and body site). (2) Methods: We defined a semi-quantitative score for the degree of AE ranging from 0 = none to 3 = total loss of elastic fibers (basophilic degeneration) and multiplied it by the perilesional vertical extent (depth), measured histometrically (tumor-associated elastosis grade (TEG)). We matched the TEG of n = 595 melanocytic lesions from 559 patients with their clinical variables. (3) Results: The TEG was correlated with age and UV-exposed body sites. Furthermore, the TEG was significantly higher in LMM than in all other types of melanomas and the TEG in NMM was higher than in SSM, irrespective of patient age and tumor site. (4) Conclusions: High cumulative UV exposure is more strongly associated with LMM and NMM than with other melanoma subtypes.},
keywords = {M8},
pubstate = {published},
tppubtype = {bachelorthesis}
}
Luo,; K.,; Liu,; S.,; Fu,; X.,; Du,; X.,; Hu,; J.,; Luo,; L.,; Fa,; C.,; Wu,; R.,; Li,; L.,; & Xu,; C.,
M8 Microscope and Scanner Unveils the Auxin-PLT5 Signaling Cascade in Wood Fiber Development
With the help of M8 microscope and scanner, this study delves into the intricate role of auxin, a crucial phytohormone enriched in the vascular cambium, in regulating wood formation in trees. Unraveling the molecular mechanisms, the research highlights the transcription factor PLETHORA 5 (PLT5), specifically activated by auxin signaling in the vascular cambium. PLT5 emerges as a key regulator, influencing cell expansion and fiber lignification in poplar. Genetic experiments underscore the noncell-autonomous nature of auxin signaling regulation from the vascular cambium, emphasizing the indispensable role of PLT5 protein mobility in mediating this process. Remarkably, PLT5 proteins inhibit the initiation of fiber cell wall thickening by directly repressing SND1 genes. The study unveils a sophisticated model wherein the auxin-PLT5 signaling cascade intricately fine-tunes wood fiber development in poplar by regulating the thickening of fiber cell walls.
Result: The 7th, 8th, and 9th internodal segments of both wild-type (WT) and transgenic poplar plants underwent sectioning. Subsequently, these sections were stained with 0.05% (w/v) toluidine blue for 5 minutes or 1.0% (w/v) phloroglucinol for 15 seconds after a 1-minute dissociation in 40% (v/v) hydrochloric acid (HCl). The resulting samples were then captured using M8 microscope and a slide scanner for documentation.
@article{nokey,
title = {The auxin-PLETHORA 5 module regulates wood fibre development in poplar in a non-cell-autonomous manner},
author = {Luo and K. and Liu and S. and Fu and X. and Du and X. and Hu and J. and Luo and L. and Fa and C. and Wu and R. and Li and L. and & Xu and C.},
url = {https://www.researchsquare.com/article/rs-3477891/v1},
year = {2023},
date = {2023-11-03},
journal = {Research Square Platform LLC},
abstract = {Auxin, as a vital phytohormone, is enriched in the vascular cambium, playing a crucial role in regulating wood formation in trees. Despite its significance, the molecular mechanisms underlying the influence of auxin on wood development remain elusive. In this study, we report a transcription factor, PLETHORA 5 (PLT5), whose expression was specifically activated by auxin signalling in the vascular cambium. PLT5 was found to regulate cell expansion and lignification of fibres in poplar. Genetic experiments confirmed the noncell-autonomous regulation of auxin signalling from the vascular cambium and revealed the necessity of PLT5 protein mobility to mediate this process. Remarkably, PLT5 proteins specifically inhibit the initiation of fibre cell wall thickening by directly repressing SND1 genes. This study unveils a sophisticated model wherein the auxin-PLT5 signalling cascade intricately regulates wood fibre development in poplar by fine-tuning the thickening of fibre cell walls.
},
keywords = {M8},
pubstate = {published},
tppubtype = {article}
}
Elmas, Hatice; Önal, Binnur; Steurer, Stefan; Hantzsch-Kuhn, Birgit; Claussen, Martin; Mehdi, Elnur; Ince, Ümit; Rabe, Klaus F.; Sauter, Guido; Welker, Lutz
Advanced Cytological Evaluation Research Uses iO:M8 Digital Live Microscope in Real-time Endoscopic Biopsy Diagnostics
The dynamic field of cytopathology has undergone a significant digital transformation, addressing challenges in image quality, scanning speed, and storage. Employing iO:M8 digital live microscopes as a pivotal technological asset, this study explores the efficacy of motorized real-time cytology in endoscopic biopsy diagnostics. Online evaluation procedures, facilitated by remote control and transmission technologies, bridge spatial gaps between medical units, enabling rapid cytological analyses at scale. The research, conducted on 239 patients at LungenClinic Großhansdorf, focuses on recording time efficiency and assessing sensitivity and specificity through rapid remote online evaluation. The study aims to showcase the transformative potential of advanced microscopy in enhancing the speed and accuracy of cytological assessments, offering valuable insights into the future of endoscopic biopsy diagnostics.
Result: Image evaluation was conducted with a dedicated monitor at each site, with specimens stained in real-time during the ongoing endoscopic examination. Researchers then utilized the iO:M8 Digital Live Microscope during the initial viewing that occurred upon the establishment of a telephone or internet connection. Collaborative discussions on findings and evaluations took place between clinically and morphologically active colleagues via telephone, facilitating seamless communication.
@article{nokey,
title = {Rapid Remote Online Evaluation in Endoscopic Diagnostics: An Analysis of Biopsy-Proven Respiratory Cytopathology},
author = {Hatice Elmas and Binnur Önal and Stefan Steurer and Birgit Hantzsch-Kuhn and Martin Claussen and Elnur Mehdi and Ümit Ince and Klaus F. Rabe and Guido Sauter and Lutz Welker},
url = {https://www.mdpi.com/2075-4418/13/21/3329},
doi = {10.3390/diagnostics13213329},
year = {2023},
date = {2023-10-28},
urldate = {2023-10-28},
journal = {Diagnostics},
volume = {13},
issue = {21},
abstract = {Background: This prospective study assesses the use of rapid remote online cytological evaluation for diagnosing endoscopical achieved biopsies. It focuses on its effectiveness in identifying benign and malignant conditions using digital image processing. Methods: The study was conducted between April 2021 and September 2022 and involved analyses of 314 Rapid Remote Online Cytological Evaluations in total (154 imprint cytologies, 143 fine needle aspirations and 17 brush cytologies) performed on 239 patients at the LungenClinic Grosshansdorf. During on-site evaluation via telecytology, the time requirement was recorded and the findings were compared with the cyto-/histological and final diagnoses. Results: By means of rapid remote online evaluation, findings of 86 cytological benign, 190 malignant and 38 unclear diagnoses were recorded (Ø assessment time, 100 s; range, 11–370 s). In 27 of the 37 specimens with unclear diagnoses, the final findings were malignant tumours and only 6 were benign changes. The diagnosis of another 4 of these 37 findings remained unclear. Excluding these 37 specimens, rapid remote online evaluation achieved a sensitivity of 90.5% with a specificity of 98.5% and a correct classification rate of 92.4% with regard to the final diagnosis of all cases. As expected, an increase in the sensitivity rate for the cytological detection of malignant tumours (76.1% vs. 92.5%) was found especially in fine-needle aspirations. Conclusions: Rapid remote online analysis allows the fast quantitative and qualitative evaluation of clinically obtained cytological specimens. With a correct classification rate of more than 93%, sampling deficiencies can be corrected promptly and diagnostic and therapeutic approaches can be derived.},
keywords = {iO:M8, Streaming Software},
pubstate = {published},
tppubtype = {article}
}
He,; R.-R.,; Luo,; X.,; Li,; Z.-C.,; Li,; D.-D.,; Li,; Z.-X.,; Gong,; H.-B.,; Yan,; C.-Y.,; Huang,; R.-T.,; Feng,; Y.,; Chen,; S.,; Cao,; Y.-F.,; Liu,; M.,; Wang,; R.,; Huang,; F.,; Sun,; W.-Y.,; Kurihara,; H.,; Duan,; W.-J.,; Liang,; L.,; Jin,; W.,; Wu, …; Y.-P.,
M8 Microscope and Scanner Uncovers Crucial Element in Ferroptosis-based Cancer Treatments
The use of the M8 microscope and scanner revealed a critical attribute in ferroptosis-based treatments. While ferroptosis holds immense potential against cancer, the lack of cell-specific ferroptosis inducers results in indiscriminate phospholipid peroxidation across tumor and non-tumor cells, limiting its safety and efficacy. Previous research uncovered that macrophages could engulf ferroptotic cells via Toll-like receptor 2 (TLR2). Expanding on this, the current study elucidates a profound mechanism: phospholipid peroxidation in macrophages impairs their ability to eliminate ferroptotic tumor cells, fostering tumor resistance to ferroptosis therapy. This impairment stems from the accumulation of phospholipid peroxidation within the macrophage endoplasmic reticulum (ER), disrupting TLR2 trafficking to the plasma membrane. Consequently, ER-retained TLR2 recruits E3 ligase MARCH6, initiating proteasome-dependent degradation. Notably, the analysis identifies SAPE-OOH as a crucial mediator in this process.
Result: Tumor tissues were processed into 4 μm sections. Macrophage phagocytosis was assessed using anti-F4/80 antibodies and HRP-conjugated secondary antibodies. Following hematoxylin staining, the samples were interpreted using the M8 microscope and scanner.
@article{nokey,
title = {Phospholipid peroxidation in macrophage confers tumor resistance by suppressing phagocytic capability towards ferroptotic cells},
author = {He and R.-R. and Luo and X. and Li and Z.-C. and Li and D.-D. and Li and Z.-X. and Gong and H.-B. and Yan and C.-Y. and Huang and R.-T. and Feng and Y. and Chen and S. and Cao and Y.-F. and Liu and M. and Wang and R. and Huang and F. and Sun and W.-Y. and Kurihara and H. and Duan and W.-J. and Liang and L. and Jin and W. and … Wu and Y.-P.},
url = {https://assets.researchsquare.com/files/rs-3396037/v1_covered_97dac08c-b002-45c1-827d-bef7d831e607.pdf?c=1697741000},
doi = {10.21203/rs.3.rs-3396037/v1},
year = {2023},
date = {2023-10-19},
journal = {Research Square Platform LLC},
abstract = {Ferroptosis holds significant potential for application in cancer therapy. However, ferroptosis inducers are not well cell-specific and can cause phospholipid peroxidation in both tumor and non-tumor cells. This limitation greatly restricts the use of ferroptosis therapy as a safe and effective anticancer strategy. Our previous study demonstrated that macrophages can engulf ferroptotic cells through Toll-like receptor 2 (TLR2). Despite this advancement, the precise mechanism by which phospholipid peroxidation in macrophages affects their phagocytotic prowess during treatment of tumors with ferroptotic agents is still unknown. Here, we determined that phospholipid peroxidation in macrophages impaired their ability to eliminate ferroptotic tumor cells by phagocytosis, ultimately fostering tumor resistance to ferroptosis therapy. Mechanistically, the accumulation of phospholipid peroxidation in the macrophage endoplasmic reticulum (ER) repressed TLR2 trafficking to plasma membrane and caused its retention in the ER by disrupting the interaction between TLR2 and its chaperone CNPY3. Subsequently, this ER-retained TLR2 recruited E3 ligase MARCH6 and initiated the proteasome-dependent degradation. Using phospholipidomics, we identified 1-steaoryl-2-15-HpETE-sn-glycero-3-phosphatidylethanolamine (SAPE-OOH) as the crucial mediator of these effects. Conclusively, this discovery elucidates a novel molecular mechanism underlying macrophage phospholipid peroxidation-induced tumor resistance to ferroptosis therapy and highlights the TLR2-MARCH6 axis as a potential therapeutic target for cancer therapy.},
keywords = {M8},
pubstate = {forthcoming},
tppubtype = {article}
}