Scientific Publications

Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.

53 entries « 1 of 6 »

Wang, Dongxu; Ren, Jing; Li, Jiping; Li, Xiuying; Ying, Jinchi; Jiang, Tiantian; Wang, Zhen; Pan, Zheng; Guo, Qianqian; Li, Chunyi; Zhang, Guokun

M8 Microscope and Scanner Utilized to Explore Deer Antler Stem Cell Conditioned Media\'s Potential in Alleviating Type 1 Diabetes via NF-κB Pathway Inhibition

A study, using the M8 microscope and slide scanner, delved into the impact of Type 1 diabetes mellitus (T1D) on human health. Type 1 diabetes mellitus (T1D) poses a significant threat to human health, primarily due to absolute insulin deficiency, leading to elevated blood glucose levels and potentially life-threatening complications such as liver injury. Mesenchymal stem cell (MSC) transplantation has emerged as a promising treatment for T1D and its associated liver injuries, leveraging the regenerative and immunomodulatory properties of MSCs. The study explored the therapeutic potential of conditioned medium from deer antler stem cells (AnSC-CM) in T1D and diabetic liver injuries. Results indicate that AnSC-CM not only alleviates T1D symptoms but also mitigates T1D-induced liver injury, outperforming bone marrow MSC-conditioned medium (BMSC-CM). Mechanistic insights suggest that the therapeutic effects of AnSC-CM may be mediated through targeting the NF-κB signaling pathway. The research proposes a novel strategy utilizing alternative stem cell conditioned mediums for effective treatment of T1D and associated liver injuries in clinical settings.

Result: Research findings demonstrated that AnSC-CM effectively relieved symptoms associated with T1D, including decreased body weight, elevated blood glucose levels, islet lesions, and diminished insulin secretion. Additionally, AnSC-CM treatment exhibited notable improvements in liver function and the mitigation of T1D-induced liver injury in mice. The therapeutic efficacy of AnSC-CM surpassed that of BMSC-CM. Analysis of underlying mechanisms unveiled significant downregulation of the NF-κB signaling pathway in both pancreatic and liver tissues by AnSC-CM. These results suggest that AnSC-CM\'s therapeutic effects on T1D and associated liver injury induced by STZ may be attributed to its modulation of the NF-κB signaling pathway.

Abstract | Links | Tags: M8

Front. Biosci. (Landmark Ed) | 2024-03-11

Hansen,; E.,; Wang,; M.,; Rolling,; C.,; & Holaska,; M., J.

PreciPoint Slide Scanning Microscope Unveils the Invasive Phenotype of MCF7 Cells under Emerin Deficiency

Using PreciPoint’s slide scanning microscope, researchers investigated how cancer cells migrate through blood vessels during metastasis. They observed that cancer cells adapt to pass through narrow gaps in the endothelium by altering their nuclei, which become more deformable. The study focused on the role of emerin protein in this process. Researchers found that invasive breast cancer cells, which have lower levels of emerin, exhibit smaller and misshapen nuclei compared to non-cancerous cells. This reduction in emerin was associated with higher rates of metastasis. To validate their hypothesis, the researchers manipulated emerin levels in noninvasive MCF7 cells and observed similar changes in nucleus size and shape, leading to impaired cell migration. This effect was consistent when emerin levels were reduced in invasive breast cancer cells. Furthermore, the analysis of breast cancer patient samples revealed a negative correlation between emerin expression and cancer invasiveness, suggesting its potential as a biomarker for tumor progression. The findings indicate that emerin loss plays a crucial role in promoting invasive transformation, highlighting its significance in cancer metastasis.

Result: To investigate how reducing emerin levels affects MCF7 cells, researchers created various MCF7 cell lines. These cell lines were engineered to produce one of three distinct emerin shRNA sequences labeled as A, B, or C, alongside a control group that expressed a scrambled shRNA sequence obtained from Genecopoeia. Immunohistochemistry and tissue microarray analyses were conducted on these cell lines. For the tissue microarray analysis, tissue microarrays underwent deparaffinization and rehydration procedures. Following additional tissue treatments, images were captured utilizing the Precipoint slide scanning microscope.

Abstract | Links | Tags: Fritz, M8

Cold Spring Harbor Laboratory | 2024-02-24

Yu, Jing; Gao, Boyuan; Li, Danning; Li, Shuang; Chiang, Vincent L.; Li, Wei; Zhou, Chenguang

M8 Microscope Reveals PtrLBD39 Overexpression Hinders Primary and Secondary Growth in Populus Trichocarpa

Researchers used M8 microscope and scanner to investigate the mechanisms underlying primary and secondary growth in trees, crucial for height and stem diameter increments, respectively, which are essential for woody biomass production. They discovered PtrLBD39, a transcription factor highly specific to stem phloem in Populus trichocarpa. Through ectopic expression of PtrLBD39 in P. trichocarpa, researchers observed a significant retardation in both primary and secondary growth. Their comprehensive analysis, including RNA-seq, ChIP-seq, and weighted gene co-expression network analysis (WGCNA), unveiled PtrLBD39\'s role in regulating transcription factors associated with vascular tissue development, wood formation, hormonal signaling, and enzymes responsible for wood components. This regulatory function resulted in growth inhibition, reduced fibrocyte secondary cell wall thickness, and diminished wood production. The study suggests that PtrLBD39 acts as a repressor, affecting both primary and secondary growth when ectopically expressed in P. trichocarpa.

Result: The stem segments, after treatment, underwent cutting into 10-micrometer sections and staining with solutions containing 5% Safranin O, 1.25% Fast Green, and 0.1% Toluidine Blue. Subsequently, micrographs of the stem sections were obtained using an M8 digital microscope and scanner. The findings of the study suggest that PtrLBD39 functions as a suppressor, impacting both primary and secondary growth upon its ectopic expression in P. trichocarpa.

Abstract | Links | Tags: M8

International Journal of Molecular Sciences | 2024-02-12

Rusche,; D.,; Englert,; N.,; Runz,; M.,; Hetjens,; S.,; Langner,; C.,; Gaiser,; T.,; & Weis,; C.-A.,

M8 Microscope and Scanner Assists Researchers in Exploring the Challenges of Detecting Tumor Budding in Colorectal Carcinoma Histology

In colorectal carcinoma (CRC) research, the M8 microscope and scanner played a pivotal role in a study aimed at predicting post-surgery treatment requirements by discerning crucial tumor features within whole slide images of solid tumors. Addressing this challenge with a small CRC dataset, researchers explored two distinct approaches. Firstly, a conventional tile-level training method was examined, incorporating various data augmentation techniques to counter the memorization effect in a noisy label setting. Secondly, a multi-instance learning (MIL) approach at the case level was explored, adapting data augmentation to prevent over-fitting in the context of limited data. The tile-level strategy proved ineffective due to a scarcity of informative image tiles per case, while the MIL approach, coupled with post-feature vector creation data augmentation, successfully predicted nodal status based on expert-derived budding scores for these cases.

Result: The dataset consisted of 29 whole slide images (WSIs), including 21 for tumors, one each for high-grade and low-grade intra-epithelial neoplasia (IEN), five for ulcerative colitis, and one for healthy tissue. The entire tissue sections were scanned and saved in the .svs format for further analysis using the M8 microscope and scanner. The study demonstrated the effectiveness of the MIL method in identifying predictive factors such as tumor budding, even within the constraints of a limited dataset size by incorporating data augmentation techniques.

Abstract | Links | Tags: M8

Applied Sciences | 2024-01-22

Stallmeyer,; B.,; Bühlmann,; C.,; Stakaitis,; R.,; Dicke,; A.-K.,; Ghieh,; F.,; Meier,; L.,; Zoch,; A.,; MacLeod,; M., D.; Steingröver,; J.,; Okutman,; Ö.,; Fietz,; D.,; Pilatz,; A.,; Escamilla,; R., A.; Xavier,; M.,; Ruckert,; C.,; Persio,; D., S.; Neuhaus,; N.,; Gurbuz,; S., A.; Şalvarci,; A.,; Tüttelmann, …; F.,

O8 Oil Microscope and Scanner Reveals Inherited Defects of piRNA Biogenesis Leading to Transposon De-Repression, Impaired Spermatogenesis, and Human Male Infertility

In an exploration of the genetic intricacies underlying piRNA dysfunction in humans, researchers leveraged the O8 oil digital microscope and slide scanner. The study delved into the critical functions of Piwi-interacting RNAs (piRNAs) in transposon silencing, germ cell maturation, and male fertility. Examining 39 infertile men with biallelic variants in 14 piRNA pathway genes, including novel candidates like PIWIL1 and GTSF1, the research uncovered distinct testicular phenotypes, ranging from complete germ cell loss to the production of abnormal spermatozoa. Notably, impaired piRNA biogenesis in spermatogonia led to transposon de-silencing, validated by elevated LINE1 expression. The study also revealed co-dependencies within the human piRNA pathway, as the abolished expression of both encoded proteins and additional piRNA factors contributed to spermatogenic failure. These findings establish the disrupted piRNA pathway as a significant cause of human infertility, providing crucial insights into transposon silencing in male germ cells.

Result: Testicular biopsies from individuals in the MERGE cohort and control subjects were acquired through testicular sperm extraction (TESE) procedures or histological assessment. These biopsies were fixed in Bouin’s solution overnight, followed by multiple processes. A the end, the processed slides were examined and documented using the O8 oil digital microscope and scanner. The study unveiled intricate co-dependencies within the human piRNA pathway. The study also demonstrated that the absence of expression in both the encoded proteins and additional piRNA factors played a significant role in contributing to spermatogenic failure.

Abstract | Links | Tags: O8

Research Square Platform LLC. | 2024-01-09

Shi,; H.,; Yuan,; X.,; Liu,; G.,; & Fan,; W.,

M8 Digital Microscope and Slide Scanning Used for Enhanced Histological Analysis of Wound Tissue

Utilizing the M8 microscope and scanner, this study harnessed the power of bioinformatics and machine learning to scrutinize microarray data sourced from the Gene Expression Omnibus (GEO) database, pinpointing crucial genes linked to Diabetic Foot Ulcers (DFU). The investigation employed GSE68183 and GSE80178 datasets as the training set, employing advanced techniques such as LASSO and SVM-RFE machine learning models through the glmnet and e1071 packages, respectively. Model validation ensued using both the training set and an independent validation set (GSE134431). Subsequent enrichment analyses, encompassing GSEA and GSVA, were applied to the identified genes. Immune functional and immune-related analyses were conducted to unravel the biological significance. The study culminated in a validation phase, employing immunohistochemistry (IHC) to corroborate the significance of the identified model genes.

Result: After preservation in 4% paraformaldehyde for 48 hours, the wound tissue underwent conventional paraffin embedding and sectioning procedures. Subsequently, staining procedures were employed, including hematoxylin and eosin, along with Masson’s trichrome stain, to enhance visualization of tissue characteristics. The stained tissues were meticulously scrutinized using a state-of-the-art M8 digital microscope and slide scanning device, ensuring precise and detailed examination of the histological features.

Abstract | Links | Tags: M8

Journal of Inflammation Research | 2023-12-19

Drexler,; K.,; Zenderowski,; V.,; Schreieder,; L.,; Koschitzki,; K.,; Karrer,; S.,; Berneburg,; M.,; Haferkamp,; S.,; & Niebel,; D.,

PreciPoint M8 Microscope and Scanner Used to Study Melanoma Variances

Researchers used the M8 microscope, scanner, and ViewPoint Software to delve into the distinct connective tissue alterations within sun-exposed skin due to prolonged sun exposure. The escalation in sun exposure and resultant sunburns leads to heightened mole formation, alongside an increased risk of both melanomas and non-melanoma skin cancers. The inquiry into the disparity between chronic sun damage and intermittent exposure raises uncertainties about their specific influence on individual melanoma susceptibility. Investigating correlations between tissue modifications, melanoma subtypes, and the body sites exposed to varying sunlight conditions, findings reveal diverse tissue changes proximal to moles and melanomas, irrespective of patient age and tumor site. This discovery elucidates the intricate biological repercussions of sunlight on pigment cells, the precursors to moles and melanomas, and also underscores the imperative to discern nuances among melanoma subtypes for a comprehensive understanding of their etiology.

Result: The photomicrographs were taken after scanning the slides using a PreciPoint M8 microscope and scanner with the ViewPointLight software for imaging. The pivotal revelation of the study lies in the discernible contrast in the depth of actinic elastosis (AE) and tumor-associated elastosis grade (TEG) layers between superficial spreading melanoma (SSM) and nodular malignant melanoma (NMM). Specifically, the mean thickness of both AE and TEG was markedly lower in SSM compared to NMM. The study also affirmed a correlation between chronic solar damage (CSD) and lentigo maligna melanoma (LMM), distinguishing it from other clinical subtypes of melanomas.

Abstract | Links | Tags: M8

Cancers | 2023-12-19

Luo,; K.,; Liu,; S.,; Fu,; X.,; Du,; X.,; Hu,; J.,; Luo,; L.,; Fa,; C.,; Wu,; R.,; Li,; L.,; & Xu,; C.,

M8 Microscope and Scanner Unveils the Auxin-PLT5 Signaling Cascade in Wood Fiber Development

With the help of M8 microscope and scanner, this study delves into the intricate role of auxin, a crucial phytohormone enriched in the vascular cambium, in regulating wood formation in trees. Unraveling the molecular mechanisms, the research highlights the transcription factor PLETHORA 5 (PLT5), specifically activated by auxin signaling in the vascular cambium. PLT5 emerges as a key regulator, influencing cell expansion and fiber lignification in poplar. Genetic experiments underscore the noncell-autonomous nature of auxin signaling regulation from the vascular cambium, emphasizing the indispensable role of PLT5 protein mobility in mediating this process. Remarkably, PLT5 proteins inhibit the initiation of fiber cell wall thickening by directly repressing SND1 genes. The study unveils a sophisticated model wherein the auxin-PLT5 signaling cascade intricately fine-tunes wood fiber development in poplar by regulating the thickening of fiber cell walls.

Result: The 7th, 8th, and 9th internodal segments of both wild-type (WT) and transgenic poplar plants underwent sectioning. Subsequently, these sections were stained with 0.05% (w/v) toluidine blue for 5 minutes or 1.0% (w/v) phloroglucinol for 15 seconds after a 1-minute dissociation in 40% (v/v) hydrochloric acid (HCl). The resulting samples were then captured using M8 microscope and a slide scanner for documentation.

Abstract | Links | Tags: M8

Research Square Platform LLC | 2023-11-03

Elmas, Hatice; Önal, Binnur; Steurer, Stefan; Hantzsch-Kuhn, Birgit; Claussen, Martin; Mehdi, Elnur; Ince, Ümit; Rabe, Klaus F.; Sauter, Guido; Welker, Lutz

Advanced Cytological Evaluation Research Uses iO:M8 Digital Live Microscope in Real-time Endoscopic Biopsy Diagnostics

The dynamic field of cytopathology has undergone a significant digital transformation, addressing challenges in image quality, scanning speed, and storage. Employing iO:M8 digital live microscopes as a pivotal technological asset, this study explores the efficacy of motorized real-time cytology in endoscopic biopsy diagnostics. Online evaluation procedures, facilitated by remote control and transmission technologies, bridge spatial gaps between medical units, enabling rapid cytological analyses at scale. The research, conducted on 239 patients at LungenClinic Großhansdorf, focuses on recording time efficiency and assessing sensitivity and specificity through rapid remote online evaluation. The study aims to showcase the transformative potential of advanced microscopy in enhancing the speed and accuracy of cytological assessments, offering valuable insights into the future of endoscopic biopsy diagnostics.

Result: Image evaluation was conducted with a dedicated monitor at each site, with specimens stained in real-time during the ongoing endoscopic examination. Researchers then utilized the iO:M8 Digital Live Microscope during the initial viewing that occurred upon the establishment of a telephone or internet connection. Collaborative discussions on findings and evaluations took place between clinically and morphologically active colleagues via telephone, facilitating seamless communication.

Abstract | Links | Tags: iO:M8, Streaming Software

Diagnostics | 2023-10-28

He,; R.-R.,; Luo,; X.,; Li,; Z.-C.,; Li,; D.-D.,; Li,; Z.-X.,; Gong,; H.-B.,; Yan,; C.-Y.,; Huang,; R.-T.,; Feng,; Y.,; Chen,; S.,; Cao,; Y.-F.,; Liu,; M.,; Wang,; R.,; Huang,; F.,; Sun,; W.-Y.,; Kurihara,; H.,; Duan,; W.-J.,; Liang,; L.,; Jin,; W.,; Wu, …; Y.-P.,

M8 Microscope and Scanner Uncovers Crucial Element in Ferroptosis-based Cancer Treatments

The use of the M8 microscope and scanner revealed a critical attribute in ferroptosis-based treatments. While ferroptosis holds immense potential against cancer, the lack of cell-specific ferroptosis inducers results in indiscriminate phospholipid peroxidation across tumor and non-tumor cells, limiting its safety and efficacy. Previous research uncovered that macrophages could engulf ferroptotic cells via Toll-like receptor 2 (TLR2). Expanding on this, the current study elucidates a profound mechanism: phospholipid peroxidation in macrophages impairs their ability to eliminate ferroptotic tumor cells, fostering tumor resistance to ferroptosis therapy. This impairment stems from the accumulation of phospholipid peroxidation within the macrophage endoplasmic reticulum (ER), disrupting TLR2 trafficking to the plasma membrane. Consequently, ER-retained TLR2 recruits E3 ligase MARCH6, initiating proteasome-dependent degradation. Notably, the analysis identifies SAPE-OOH as a crucial mediator in this process.

Result: Tumor tissues were processed into 4 μm sections. Macrophage phagocytosis was assessed using anti-F4/80 antibodies and HRP-conjugated secondary antibodies. Following hematoxylin staining, the samples were interpreted using the M8 microscope and scanner.

Abstract | Links | Tags: M8

Research Square Platform LLC | 2023-10-19

53 entries « 1 of 6 »