Scientific Publications
Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.
PreciPoint M8 Microscope and Scanner Used to Study Melanoma Variances
Researchers used the M8 microscope, scanner, and ViewPoint Software to delve into the distinct connective tissue alterations within sun-exposed skin due to prolonged sun exposure. The escalation in sun exposure and resultant sunburns leads to heightened mole formation, alongside an increased risk of both melanomas and non-melanoma skin cancers. The inquiry into the disparity between chronic sun damage and intermittent exposure raises uncertainties about their specific influence on individual melanoma susceptibility. Investigating correlations between tissue modifications, melanoma subtypes, and the body sites exposed to varying sunlight conditions, findings reveal diverse tissue changes proximal to moles and melanomas, irrespective of patient age and tumor site. This discovery elucidates the intricate biological repercussions of sunlight on pigment cells, the precursors to moles and melanomas, and also underscores the imperative to discern nuances among melanoma subtypes for a comprehensive understanding of their etiology.
Result: The photomicrographs were taken after scanning the slides using a PreciPoint M8 microscope and scanner with the ViewPointLight software for imaging. The pivotal revelation of the study lies in the discernible contrast in the depth of actinic elastosis (AE) and tumor-associated elastosis grade (TEG) layers between superficial spreading melanoma (SSM) and nodular malignant melanoma (NMM). Specifically, the mean thickness of both AE and TEG was markedly lower in SSM compared to NMM. The study also affirmed a correlation between chronic solar damage (CSD) and lentigo maligna melanoma (LMM), distinguishing it from other clinical subtypes of melanomas.
Cancers | 2023-12-19
M8 Microscope and Scanner Unveils the Auxin-PLT5 Signaling Cascade in Wood Fiber Development
With the help of M8 microscope and scanner, this study delves into the intricate role of auxin, a crucial phytohormone enriched in the vascular cambium, in regulating wood formation in trees. Unraveling the molecular mechanisms, the research highlights the transcription factor PLETHORA 5 (PLT5), specifically activated by auxin signaling in the vascular cambium. PLT5 emerges as a key regulator, influencing cell expansion and fiber lignification in poplar. Genetic experiments underscore the noncell-autonomous nature of auxin signaling regulation from the vascular cambium, emphasizing the indispensable role of PLT5 protein mobility in mediating this process. Remarkably, PLT5 proteins inhibit the initiation of fiber cell wall thickening by directly repressing SND1 genes. The study unveils a sophisticated model wherein the auxin-PLT5 signaling cascade intricately fine-tunes wood fiber development in poplar by regulating the thickening of fiber cell walls.
Result: The 7th, 8th, and 9th internodal segments of both wild-type (WT) and transgenic poplar plants underwent sectioning. Subsequently, these sections were stained with 0.05% (w/v) toluidine blue for 5 minutes or 1.0% (w/v) phloroglucinol for 15 seconds after a 1-minute dissociation in 40% (v/v) hydrochloric acid (HCl). The resulting samples were then captured using M8 microscope and a slide scanner for documentation.
Research Square Platform LLC | 2023-11-03
Advanced Cytological Evaluation Research Uses iO:M8 Digital Live Microscope in Real-time Endoscopic Biopsy Diagnostics
The dynamic field of cytopathology has undergone a significant digital transformation, addressing challenges in image quality, scanning speed, and storage. Employing iO:M8 digital live microscopes as a pivotal technological asset, this study explores the efficacy of motorized real-time cytology in endoscopic biopsy diagnostics. Online evaluation procedures, facilitated by remote control and transmission technologies, bridge spatial gaps between medical units, enabling rapid cytological analyses at scale. The research, conducted on 239 patients at LungenClinic Großhansdorf, focuses on recording time efficiency and assessing sensitivity and specificity through rapid remote online evaluation. The study aims to showcase the transformative potential of advanced microscopy in enhancing the speed and accuracy of cytological assessments, offering valuable insights into the future of endoscopic biopsy diagnostics.
Result: Image evaluation was conducted with a dedicated monitor at each site, with specimens stained in real-time during the ongoing endoscopic examination. Researchers then utilized the iO:M8 Digital Live Microscope during the initial viewing that occurred upon the establishment of a telephone or internet connection. Collaborative discussions on findings and evaluations took place between clinically and morphologically active colleagues via telephone, facilitating seamless communication.
Diagnostics | 2023-10-28
M8 Microscope and Scanner Uncovers Crucial Element in Ferroptosis-based Cancer Treatments
The use of the M8 microscope and scanner revealed a critical attribute in ferroptosis-based treatments. While ferroptosis holds immense potential against cancer, the lack of cell-specific ferroptosis inducers results in indiscriminate phospholipid peroxidation across tumor and non-tumor cells, limiting its safety and efficacy. Previous research uncovered that macrophages could engulf ferroptotic cells via Toll-like receptor 2 (TLR2). Expanding on this, the current study elucidates a profound mechanism: phospholipid peroxidation in macrophages impairs their ability to eliminate ferroptotic tumor cells, fostering tumor resistance to ferroptosis therapy. This impairment stems from the accumulation of phospholipid peroxidation within the macrophage endoplasmic reticulum (ER), disrupting TLR2 trafficking to the plasma membrane. Consequently, ER-retained TLR2 recruits E3 ligase MARCH6, initiating proteasome-dependent degradation. Notably, the analysis identifies SAPE-OOH as a crucial mediator in this process.
Result: Tumor tissues were processed into 4 μm sections. Macrophage phagocytosis was assessed using anti-F4/80 antibodies and HRP-conjugated secondary antibodies. Following hematoxylin staining, the samples were interpreted using the M8 microscope and scanner.
Research Square Platform LLC | 2023-10-19
M8 Microscope and Scanner Used to Investigate N-acetylcysteine’s Impact on Reactive Oxygen Species and Endothelial Cells
Interpretation of specimens through M8 microscope and scanner helped researchers develop a novel animal phlebitis model using a carbomer/VNR sustained-release gel injected around veins, inducing phlebitis efficiently. VNR, a potent antitumor drug, when mixed with stable and safe carbomer, stimulated veins locally, leading to phlebitis. This model allowed for the exploration of phlebitis pathogenesis and prevention strategies. Additionally, the study investigated N-acetylcysteine (NAC), an antioxidant that reduced reactive oxygen species levels and endothelial cell damage. NAC attenuated VNR-induced mitochondrial damage, inhibiting phlebitis occurrence. These findings suggest NAC administration alongside chemotherapy drugs could prevent or treat phlebitis, providing valuable insights for clinical practice.
Result: The paraffin-embedded sections underwent deparaffinization process to ensure proper hydration. Subsequently, the sections were stained with hematoxylin and eosin. Following staining, washing, and drying procedures, the sections were sealed using neutral balsams and interpreted using the M8 microscope and scanner.
Heliyon | 2023-10-18
M8 Microscope and Scanner Used to Research Toxic Effects of Koumine on the Early-Life Development Stage of Zebrafish
Researchers used the M8 microscope and scanner to explore the intricate world of zebrafish embryos and larvae, unveiling the profound impact of koumine, a prevalent alkaloid in Gelsemium elegans. Despite its potential in antitumor, anti-inflammatory, analgesic, and antianxiety treatments, koumine\'s medicinal promise is hindered by its high toxicity and elusive mechanism of action. Through examination, it was discovered that exposure to varying concentrations of koumine led to developmental irregularities in zebrafish, including hatching and heartbeat disruptions, shortened bodies, yolk sac edemas, and tail malformations. Behavioral studies showcased impaired escape responses, revealing the neurotoxic effects of koumine. Notably, this toxicity was attributed to alterations in acetylcholine content and acetylcholinesterase (AChE) activity without compromising motor neuron development. The research discerned that concentrations exceeding 25 mg/L pose significant threats to zebrafish, shedding light on the critical safety threshold for koumine application.
Result: Zebrafish larvae, exposed to 100 mg/L koumine, were fixed in 4% paraformaldehyde for 24 hours, dehydrated, and embedded in paraffin. Samples were stained with hematoxylin and eosin. Histological images were captured using the M8 microscope and scanner.
Toxics | 2023-10-12
O8 Oil Microscope and Scanner Enables Heart Tissue Characterization and Agrin-α-Dystroglycan Interaction Research
The O8 oil digital microscope and slide scanner was used in a study to investigate whether similar pathways for agrin-induced cardiomyocyte growth exist in humans. Mature heart muscle cells, known as cardiomyocytes, typically cannot multiply. This means that when the heart is injured, the damage is usually permanent. However, previous research has shown promising results in using a protein called agrin to promote healing in animal models, such as mice and pigs, following a heart attack. Scientists suggested that agrin might encourage the growth of new cardiomyocytes, particularly in mice, by interacting with a specific receptor on the cell surface called α-dystroglycan. To explore whether similar pathways for agrin-induced cardiomyocyte growth exist in humans and to understand how agrin interacts with α-dystroglycan and its effects on the heart\'s ability to regenerate, experts examined levels of agrin and related proteins in human heart tissue through genetic and protein analyses. Additionally, they created a purified form of agrin, known as rec.Agrin, which specifically contains the parts of the protein that interact with α-dystroglycan, to better understand this interaction and its downstream effects.
Result: Researchers observed that agrin transcripts were present in all samples examined. They found a negative correlation between agrin levels and age (p = 0.026), as well as with laminin transcripts (p = 0.023). However, no such correlation was observed with age for the other analyzed proteins. Using Western blotting and immunohistochemistry, experts confirmed the presence of agrin in all patient samples and localized its distribution in the tissue. A small-angle X-ray scattering (SAXS) analysis was used to observe the structure of rec.Agrin. It was found that rec.Agrin adopts a compact conformation, which is further condensed in the presence of calcium ions (Ca2+). Furthermore, solid-phase binding assays demonstrated that rec.Agrin tightly binds to α-DG in a calcium-dependent manner. However, when analyzing mutants, the binding of rec.Agrin to α-DG was significantly reduced in solid-phase experiments.
2023-10-10
M8 Microscope and Scanner Used to Investigate the Alleviating Effects of Antler Stem Cell Exosomes on Pulmonary Fibrosis
Researchers used the M8 microscope and scanner to explore the potential of deer antler stem cells (AnSCs) in treating pulmonary fibrosis (PF), a chronic lung disease characterized by excessive collagen deposition. Their findings revealed that administering AnSCs significantly improved the survival of PF mice, reduced lung fibrosis, collagen buildup, and myofibroblast formation. Remarkably, the effects of AnSC treatment surpassed those of adipose-derived stem cells, a common positive control. AnSC-Exos (exosomes) were as effective as AnSCs, indicating their potential in PF treatment through a paracrine mechanism. Additionally, AnSC-Exos reduced the number of pro-fibrotic M2 macrophages in lung tissues by inhibiting their recruitment, possibly by regulating specific molecules like CCL7. The study highlights the promising use of antler stem cells and their exosomes as a novel strategy for treating pulmonary fibrosis in clinical applications.
Result: Lung tissues were stained with Hematoxylin-Eosin (HE), Sirius Red, and Masson techniques. Subsequently, the stained samples were interpreted using our M8 microscope and scanner. Researchers employed the Ashcroft score method to assess pulmonary lesion development based on histological images.
Cell Death Discovery | 2023-09-28
M8 and ViewPoint Expose Muscle Tissue to rBMP-2, rTGF-β3, and/or rBMP-7 under Ex Vivo Conditions to Analyze Osteogenic and Chondrogenic Differentiation Pathways
The study aimed to decode the temporal and spatial signaling code for clinical cartilage and bone regeneration by investigating the effects of continuous growth factor combinations (BMP-2, TGF-β3, BMP-7) on muscle tissue from adult male rats. Cultured in 96-well plates with specific conditions, tissues were interpreted using the M8 microscope and scanner and the ViewPoint software. Contrary to expectations, the growth factor application led to a complex tissue response, deviating from chronological signaling cascades. Both osteogenic and chondrogenic genes exhibited upregulation, confirmed by immunohistochemical staining. These results challenge existing tissue formation paradigms. The study highlights the need to understand precise signal order and expression patterns for chondrogenesis and osteogenesis, crucial for clinical bone and cartilage regrowth treatments.
Result: Researchers performed histological and histomorphometric analyses on 144 tissue samples. The sections were processed, imaged at 40x magnification, and digitized using M8 microscope and scanner and ViewPoint software. Immunohistochemically stained sections were also imaged at 40x magnification and digitized with the same equipment.
CARTILAGE | 2023-09-15
Fritz Slide Scanner Used to Explore AMH’s Role in Assessing Ovarian Health in Female Animals and its Intriguing Variability in Healthy Cats
Fritz slide scanner helped researchers gain deeper insights into the origin of AMH and its release into the bloodstream, as they measured AMH levels in the blood of female cats undergoing routine ovariectomy surgery. Additionally, researchers examined AMH protein expression in various ovarian structures, including follicles, corpora lutea, and cysts, offering valuable insights into AMH\'s ovarian source and its circulation. The researchers made several significant discoveries during their study. One key hypothesis they explored was that Anti-Müllerian Hormone (AMH) is produced exclusively in preantral and small antral follicles. Additionally, they proposed that the concentration of AMH in the plasma directly corresponds to the count of antral follicles (AFC). In the female cat population under investigation, 35% of the subjects exhibited corpora lutea, while 57% had a plasma progesterone concentration of ≥1 ng/mL. Furthermore, 15 out of the cats (16%) were found to have follicular cysts.
Result: Ovaries were dissected, sliced, embedded in paraffin, and cut into thin sections using a microtome. These sections were placed on glass slides, dried overnight at 37°C, stained with hematoxylin and eosin, and digitally scanned at 20x magnification using Fritz slide scanner.
Theriogenology | 2023-08-30
