Scientific Publications

Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.

20 entries « 1 of 2 »

Binnur Önal Hatice Elmas, Stefan Steurer; Welker, Lutz

Advanced Cytological Evaluation Research Uses iO:M8 Digital Live Microscope in Real-time Endoscopic Biopsy Diagnostics

The dynamic field of cytopathology has undergone a significant digital transformation, addressing challenges in image quality, scanning speed, and storage. Employing iO:M8 digital live microscopes as a pivotal technological asset, this study explores the efficacy of motorized real-time cytology in endoscopic biopsy diagnostics. Online evaluation procedures, facilitated by remote control and transmission technologies, bridge spatial gaps between medical units, enabling rapid cytological analyses at scale. The research, conducted on 239 patients at LungenClinic Großhansdorf, focuses on recording time efficiency and assessing sensitivity and specificity through rapid remote online evaluation. The study aims to showcase the transformative potential of advanced microscopy in enhancing the speed and accuracy of cytological assessments, offering valuable insights into the future of endoscopic biopsy diagnostics.

Result: Image evaluation was conducted with a dedicated monitor at each site, with specimens stained in real-time during the ongoing endoscopic examination. Researchers then utilized the iO:M8 Digital Live Microscope during the initial viewing that occurred upon the establishment of a telephone or internet connection. Collaborative discussions on findings and evaluations took place between clinically and morphologically active colleagues via telephone, facilitating seamless communication.

Abstract | Links | Tags: iO:M8, Streaming Software

Diagnostics | 2023-10-28

Zoran Popovic Mei Li, Chang Chu

PreciPoint Microscope and Scanner and MicroPoint Software Helps Study the Impact of Angiopoietin-2 on Kidney Diseases

Angiopoietins, Ang-1, and Ang-2, are crucial in angiogenesis and vascular health. In kidney diseases, Ang-2 disruption leads to chronic inflammation. Researchers used PreciPoint’s microscope and scanner, and its MicroPoint Software to study angiopoietins in kidney diseases. Elevated Ang-2 in renal tissues and blood of kidney disease patients marks it as a potential biomarker. Our findings illuminate angiopoietins\' nuanced roles, guiding future research and targeted therapies. During the research, they unraveled the intricate mechanisms, opening avenues for improving diagnosis and treatment strategies in kidney diseases. 

Result: Immunohistochemistry was performed on paraffin-embedded renal biopsy samples from a healthy biopsy using a primary antibody (Angiopoietin 2 Polyclonal Antibody). Specimen images were acquired using PreciPoint microscope and scanner with objective x40/0.65 NA, which was running its MicroPoint software. 

Abstract | Links

Karger | 2023-03-14

Junling Zhang Mingyan Xu, Xuemei Lu

Tissue Microarray Analysis Detects SMARCA4 in the Nucleus of Oral Squamous Cell Carcinoma Tissue Samples with the Help of M8 Microscope and Scanner

The study investigates the role of SMARCA4, a subunit of the SWI/SNF chromatin remodeling complex, in oral squamous cell carcinoma (OSCC) with the help of M8 microscope and scanner. Using a tissue microarray, elevated SMARCA4 expression was observed in OSCC tissues. Upregulated SMARCA4 led to enhanced migration, invasion in vitro, and increased tumor growth in vivo, attributed to the promotion of epithelial–mesenchymal transition (EMT). Bioinformatic analysis and luciferase assays identified miR-199a-5p as a regulator of SMARCA4. Mechanistic studies revealed that miR-199a-5p-controlled SMARCA4 facilitated tumor cell invasion and metastasis through EMT regulation. This study sheds light on the miR-199a-5p-SMARCA4 axis, elucidating its involvement in OSCC progression. These findings provide crucial insights into SMARCA4\'s role in OSCC and its underlying mechanisms, offering potential therapeutic avenues. 

Result: Immunohistochemistry (IHC) staining was conducted on a human oral squamous cell carcinoma tissue microarray, containing 40 OSCC tissues and 8 normal oral mucosa tissues. The resulting images were obtained through M8 microscope and scanner and were examined independently by a pathologist. 

Abstract | Links | Tags: M8

International Journal of Molecular Sciences | 2023-03-01

Hao Dong Meng Li, Jiyuan Li

M8 Microscope and Scanner Helps Researchers Study Water Retention Efficiency of OE-PtrVCS2 in Trees During Drought Stress

With the aid of the M8 microscope and canner and its Streaming Software, researchers delved into the molecular intricacies of drought resistance in forest trees. They focused on PtrVCS2, a gene in Populus trichocarpa (Black Cottonwood), to unravel its role in drought adaptability. Overexpression of PtrVCS2 resulted in compact growth, smaller stem vessels, and robust drought resistance. Stomatal movement experiments demonstrated reduced stomatal apertures in PtrVCS2 transgenics under drought conditions. Through RNA-seq analysis, researchers identified PtrVCS2\'s influence on genes governing stomatal dynamics and cell wall biosynthesis, such as PtrSULTR3;1-1, PtrFLA11-12, and PtrPR3-3. The transgenic plants exhibited enhanced water use efficiency during prolonged drought stress. The research showcases PtrVCS2 as a key player in enhancing adaptability and resistance to drought in P. trichocarpa, offering insights into strategic engineering for drought-resistant tree species. 

Result: To conduct the research successfully, researchers used M8 microscope and scanner and the Streaming Software available with it. They used it to measure the size and area of vessels harvested from the 5th–10th internodes of OE-PtrVCS2 transgenics and wild-type plants.

Abstract | Links | Tags: M8

Molecular Sciences | 2023-02-24

Bernadett Kurz Philipp von Breitenbuch, Susanne Wallner

Highly Expressed pH-Sensitive GPCRs Found in Peritoneal Carcinomatosis Tissue Samples Obtained from Colorectal Cancer Using the M8 and ViewPoint 

Researchers utilized the M8 microscope and scanner and Viewpoint virtual microscopy viewing software to investigate the expression of multiple G protein-coupled receptors (GPCRs) in peritoneal carcinomatosis tissue samples from colorectal cancer. The research studied the expressions of GPR4, GPR65, GPR68, GPR132, and GPR151. GPR4 was just expressed weakly in 30% of samples and expression was significantly reduced as compared to GPR56, GPR132, and GPR151. The study revealed that GPR151, GPR65, and GPR132 were highly expressed in these samples. These receptors are pH-sensitive, meaning they are activated by protonation and are implicated in a variety of biological processes, including cancer cell proliferation, metastasis, angiogenesis, apoptosis, immune cell function, and inflammation. Due to their involvement in such diverse processes, the effects of these GPCRs can sometimes be opposing to one another.

Result: Hematoxylin and eosin stained-tissue samples were fixed on slides which were scanned with M8 microscope and scanner. The digital images were later edited online with the help of ViewPoint virtual microscopy viewing software. 

Abstract | Links | Tags: M8, ViewPoint

Journal of Clinical Medicine | 2023-02-23

Yingying Yang Lu Ding, Zeyu Wang

M8 Microscope and Scanner Used to Study the Pharmacological Impact of QM against PF in COVID -19 Patients

The coronavirus, also known as COVID-19, ravaged the world with its deadly spread in 2019. Pulmonary fibrosis (PF) occurred in severe COVID-19 patients, and signs of PF were found in the anatomical examination of the dead COVID -19 patients. On the treatment front, medical experts used Qimai Feiluoping Decoction (QM), a traditional Chinese medicine (TCM) formula, to improve the syndromes of phlegm-stasis in channels in convalescent COVID-19 patients and recommended it in a rehabilitation program for functional disorders of discharged patients. Researchers studied the pharmacological action of QM against PF from the perspective of imaging, pathological staining, and molecular mechanisms and identified possible active components.

Result: The immunohistochemistry (IHC) staining was performed to analyze the expression of PF-related biomarkers in lung tissues. After staining, the images of each section were acquired through our M8 Microscope and Scanner.

Abstract | Links | Tags: M8

Elsevier | 2023-02-11

Capellmann, Sandro; Sonntag, Roland; Schüler, Herdit; Meurer, Steffen K; Gan, Lin; Kauffmann, Marlies; Horn, Katharina; Königs-Werner, Hiltrud; Weiskirchen, Ralf; Liedtke, Christian; Huber, Michael

Fritz Slide Scanner Helps Researchers Understand the Cause of the Transformation of Murine PMCs in Connective Tissue

Researchers used our Fritz slide scanner in a study aimed at understanding the cause of the transformation of murine peritoneum-derived mast cells (PMCs) in connective tissue. Mast cells play a crucial role in both cancer progression and the immune response to infections by viruses and bacteria. They support angiogenesis, degradation of the extracellular matrix, and the transition from epithelial to mesenchymal cells. PMCs, which are mast cells isolated from the peritoneum, were the focus of the study. The goal was to determine why these PMCs were undergoing transformation.

Result: Researchers used our Fritz Slide Scanner equipped with a 40x objective and our MicroPoint software for the microscopic review and interpretation of the MGG-stained MCs. To view or transfer data, researchers used our ViewPoint and ConvertPoint software.

Abstract | Links | Tags: Fritz, ViewPoint

bioRxiv | 2023-01-24

Dabel, Jennifer; Schneider, Florian; Wistuba, Joachim; Kliesch, Sabine; Schlatt, Stefan; Neuhaus, Nina

Our Microscope and ViewPoint in Use for Research in Fertility Feasibility in Transwomen

The study aimed to evaluate the feasibility of fertility preservation in pre-pubertal individuals undergoing gender-affirming hormone therapy. The established method of cryopreservation of immature testicular tissue, containing a population of diploid spermatogonia, is utilized as a means of preserving the spermatogenic potential of these individuals. A retrospective analysis of testicular tissue from 25 selected subjects was performed, who received a comparable hormonal regimen utilizing cyproterone acetate and estrogens.

Result: For the analysis of tissue and measurement of germ cells, the researchers used our digital microscope equipped with a 60x objective. Researchers analyzed two independent MAGE A4-stained tissue sections. The samples were then viewed using ViewPoint software, which was used to identify and count all MAGE A4-positive and -negative spermatogonia within each sample.

Abstract | Links | Tags: Fritz, ViewPoint

Reproduction and Fertility | 2023-01-18

Salmah Aalmri Kholoud Alwosaibai, Miral Mashhour

M8 Microscope and Scanner and ViewPoint Software Unlocks PD-L1 Expression Activities in Ovarian Cancer Dynamics

Researchers investigated the potential of immune checkpoint inhibitors, specifically PD-L1 inhibitors, in treating ovarian cancer with the help of M8 microscope and scanner and ViewPoint software. Surprisingly, they found high PD-L1 expressions in 47.8% of ovarian cancer samples, spanning various epithelial ovarian cancer types. Contrary to expectations, high PD-L1 expression wasn\'t linked to poor prognosis. Additionally, 81% of ovarian cancer samples exhibited TILs expressing both CD8 and CD4, significantly correlating with elevated PD-L1 levels. Furthermore, high PD-L1 expression was associated with stem cell markers CD44 and LGR5, hinting at a connection between immune response, stem cells, and ovarian cancer. PD-L1 overexpression, especially in serous ovarian carcinomas, didn\'t impact overall survival rates. This finding underscores the intricate interplay between PD-L1, TILs, and cancer stem cells, suggesting that inhibiting PD-L1 with immune checkpoint inhibitors might curtail the stem cell population linked to cancer recurrence.

Result: Anti-CD8 (clone 4B11) mouse monoclonal antibody and Anti-CD4 (clone 4B12) mouse monoclonal antibody were used with a dilution ratio of 1:100 for tumor-infiltrating lymphocytes. The subsequent immunoreactivity was observed using M8 digital microscope and scanner. The specimen was further imaged and analyzed using ViewPoint Virtual Slide Viewing Software.

Abstract | Links | Tags: M8

BMC Cancer | 2023-01-05

Patrizia Moser Aleksandrs Krigers, Helga Fritsch; Freyschlag, Christian F.

M8 Microscope and Scanner Helps Understand the Role of Connexin-43 and Ki67 in Mon-glial Central Nervous System Tumors

Intercellular communication mediated by gap junctions plays an important role in the exchange of small molecules and ions, which not only serve to maintain homeostasis but also affect cell proliferation and differentiation. The protein connexin-43 (Cx43) is involved in the formation of these gap junctional channels and hemichannels. To better understand this, researchers examined the expression of Cx43 and Ki67 in formalin-fixed, paraffin-embedded samples of human brain metastases, meningiomas and neurinomas using immunohistochemistry. To comprehensively understand the functions of connexin-43 and Ki67 within non-glial central nervous system tumors, researchers used PreciPoint’s M8 digital microscope and scanner.

Result: Researchers used our M8 Microscope and Scanner in multiple instances. M8 Microscope and Scanner 200x magnification helped researchers to understand why metastases from NSCLC did not express Cx43 as opposed to the affected cortex. Additionally, samples under M8 showed that the cellular nuclei remain unstained.

Abstract | Links | Tags: M8

Sage Journals | 2023-01-01

20 entries « 1 of 2 »