Scientific Publications

Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.

59 entries « 1 of 6 »

Kuritcyn, Petr; Kletzander, Rosalie; Eisenberg, Sophia; Wittenberg, Thomas; Bruns, Volker; Evert, Katja; Keil, Felix; Ziegler, Paul K.; Bankov, Katrin; Wild, Peter; Eckstein, Markus; Hartmann, Arndt; Geppert, Carol I.; Benz, Michaela

M8 Microscope and Scanner Used in a Study to Achieve High Accuracy with Few-Shot Classification and Data Augmentation in Histopathology

Researchers used the M8 digital microscope and scanner to enhance artificial intelligence (AI) in histopathology, addressing image scarcity and data heterogeneity. They trained models with data from one scanner and site, achieving 90% accuracy on a multicenter database, matching primary data performance. EfficientNet B0 was the best for feature extraction. Prototypes from different scanners showed minimal accuracy deviation. Adapting to new tumor entities, the model classified urothelial carcinoma tissues with 93.6% accuracy using three annotations per subclass. This method supports an interactive AI system requiring few annotations and is adaptable to new tasks without extensive retraining. This demonstrates that few-shot classification with data augmentation can train robust models with minimal data. The approach allows for easy adaptation to new tasks, making it ideal for non-technical users. By reducing the need for extensive annotations and complex retraining, this method significantly improves efficiency and usability in histopathology AI applications.

Result: All tissue sections from the primary test set (30 slides) and adaptation set (9 slides) were digitized with four microscopic scanners including Precipoint’s M8 microscope and scanner. For manual digitization, a camera was mounted on a standard microscope and connected to a computer. The microscope stage was moved manually, systematically traversing the glass slide while the software stitched images from the video stream in real-time, resulting in one WSI representing the glass slide. The data set was included as a special challenge, with sub-optimal imaging conditions like saturation chosen during digitization. Each WSI was aligned to the WSI obtained by the primary scanner to transfer the annotations of the tissue classes to it.

Abstract | Links | Tags: M8

Journal of Pathology Informatics | 2024-06-06

Gao, Jia; Ren, Jing; Ye, Hanjie; Chu, Wenhui; Ding, Xuankai; Ding, Lingzhi; Fu, Yongqian

M8 Microscope and Scanner Used in Research on ZIF-8 Sericin Hydrogel for Bone Regeneration Promoting Angiogenesis and Osteogenesis

A recent study used the M8 microscope and scanner to evaluate a novel bone regeneration scaffold. Designing scaffolds with optimal biodegradability, osteogenic potential, and angiogenic properties presents a significant challenge. Thymosin beta 10 (TMSB10), known for its roles in angiogenesis and osteogenic differentiation, faces activity preservation limitations. To address this, researchers engineered ZIF-8 as a carrier for TMSB10 (TMSB10@ZIF-8), integrating it into a self-assembled sericin hydrogel. Testing the composite in a rat cranial defect model revealed successful synthesis of TMSB10@ZIF-8 with an 88.21% encapsulation efficiency. The sustained release of TMSB10 from this composite significantly enhanced tube formation in HUVECs and promoted angiogenesis in the CAM model. Additionally, it notably improved osteogenic differentiation in MC 3 T3-E1 cells. Eight weeks post-implantation, the TMSB10@ZIF-8/Sericin hydrogel group demonstrated substantial bone healing (86.77 ± 8.91%), outperforming controls.

Result: Histological observation of the skulls was conducted using M8 microscope and scanner. The skulls were first decalcified, followed by dehydration using a graded series of ethanol. Subsequently, the samples were cleared in xylene and embedded in paraffin. Tissue slices were then obtained from the central area of each defect and subjected to staining with H&E, Masson\'s trichrome, and Sirius Red for microscopic evaluation. The approach enabled detailed examination and documentation of tissue morphology and composition within the bone defects, providing crucial insights into the efficacy of the TMSB10@ZIF-8/Sericin hydrogel scaffold in promoting bone regeneration.

Abstract | Links | Tags: M8

International Journal of Biological Macromolecules | 2024-05-01

Fuentes, Mary Esmeralda; Lu, Xiaoyin; Flores, Natasha M.; Hausmann, Simone; Mazur, Pawel K.

M8 Microscope and Scanner Investigates High-Grade Pancreatic Neuroendocrine Tumors in Mice with MEN1, ATRX, and PTEN Deletion

M8 microscope and scanner was used in the research involving pancreatic neuroendocrine tumors (PanNETs) that are rare yet aggressive malignancies lacking effective therapies. Characterized by extensive heterogeneity, PanNETs often present at advanced stages, resulting in poor prognoses. Research efforts focusing on genomic landscapes have identified recurrent mutations in MEN1, ATRX, DAXX, and PTEN, critical in PanNET pathogenesis. The development of effective therapeutics is hindered by this heterogeneity and limited preclinical models. Notably, MEN1, ATRX, and PTEN deletions in mice mimic PanNET tumorigenesis, offering a promising model for translational research and therapeutic development. Understanding the roles of these mutations and associated pathways holds potential for targeted precision therapies in this challenging disease.

Result: Researchers used the M8 microscope and scanner to identify key genetic alterations in human PanNETs. They analyzed the GENIE v14.1 genomic sequencing database, revealing frequent mutations in MEN1, ATRX, DAXX, and PTEN. Co-occurring mutations in MEN1, PTEN, and ATRX or DAXX suggest their combined loss drives PanNET pathogenesis. Using MAP mutant mice (Men1LoxP/LoxP; AtrxLoxP/LoxP; PtenLoxP/LoxP; Pdx1-CreER), experts induced tumorigenesis and observed robust tumor development resembling intermediate-grade PanNETs with progressive malignancy. RNA sequencing confirmed elevated neuroendocrine and PanNET marker gene expression, aligning with human PanNET biology. Transcriptomic analysis revealed MAP model similarity to human PanNETs, distinct from other mouse models, highlighting its relevance for studying PanNET pathogenesis.

Abstract | Links | Tags: M8

Scientific Reports | 2024-04-12

Porth, Isabel

M8 Microscope and Scanner and ViewPoint Software Used to Study Trastuzumab\'s Long-term Effects in HER2-positive Advanced Gastric or Gastroesophageal Adenocarcinoma

A study, conducted with the help of M8 microscope and scanner and ViewPoint software, identified biomarkers distinguishing HER2-positive gastric cancer patients with long-term versus short-term responses to trastuzumab plus chemotherapy. Genetic analyses revealed two non-synonymous mutations in ERBB2, occurring exclusively in long-term responders. However, neither HER2 gene copy number nor other genetic alterations correlated with treatment response. Notably, patients with homogeneous HER2 protein expression had improved progression-free survival on trastuzumab-containing therapy. Evaluating PD-L1, long-term responders exhibited higher PD-L1 combined positive scores, correlating positively with progression-free survival across the cohort. PD-L1 positivity (combined positive score ≥1) was associated with enhanced progression-free survival on trastuzumab-based treatment. Bioinformatics analysis linked increased PD-L1 scores to elevated CD4+ memory T-cell levels. While genetic mutations in ERBB2 were specific to long-term responders, a homogeneous HER2 expression pattern and PD-L1 positivity emerged as potential biomarkers for improved treatment outcomes in HER2-positive gastric cancer, emphasizing the clinical importance of PD-L1 in this setting.

Result: The study used M8 microscope and scanner and ViewPoint software to identify that the PD-L1 combined positive score (CPS) is higher in long-term responders compared to short-term responders, suggesting a potential biomarker for predicting the efficacy of trastuzumab in these patients. The PD-L1 CPS was also positively correlated with progression-free survival (PFS), highlighting its potential as a prognostic marker for better outcomes under trastuzumab-based therapy. The study found that the copy number variations (CNVs) of the HER2 gene (ERBB2) did not significantly distinguish between long-term and short-term responders. However, patients with a homogeneous HER2 expression pattern showed improved PFS, suggesting that the uniformity of HER2 expression might be an important factor in the effectiveness of trastuzumab therapy. The CD4+ memory T-cells were found in higher levels in PD-L1 positive patients. Overall, the thesis suggests that PD-L1 expression and the immune cell landscape, particularly the presence of CD4+ memory T-cells, hold significant potential as indicators for better clinical outcomes in HER2-positive gastric and gastroesophageal junction cancers.

Abstract | Links | Tags: M8, ViewPoint

2024-04-11

Tang, Yuan-juan; Zhang, Zhen; Yan, Tong; Chen, Ken; Xu, Guo-fan; Xiong, Shi-qiang; Wu, Dai-qian; Chen, Jie; Jose, Pedro A.; & Jin-juan Fu, Chun-yu Zeng

M8 Microscope and Scanner Explores Irisin’s Impact on Type 1 Diabetic Cardiomyopathy

Research on diabetic cardiomyopathy (DCM) in type 1 diabetes mellitus (T1DM) was conducted with the assistance of an M8 microscope and scanner. Diabetes, particularly type 1 diabetes mellitus (T1DM), presents a significant global health challenge, with diabetic cardiomyopathy (DCM) posing a major threat to patients, characterized by cardiac dysfunction leading to morbidity and mortality. While apoptosis is recognized as a primary mechanism in DCM, recent evidence suggests a role for ferroptosis, a distinct form of cell death involving iron accumulation and lipid peroxidation. Despite emerging links between ferroptosis and DCM, understanding remains limited, especially in T1DM. Training has shown protective effects against DCM, and irisin, a myokine, has been implicated in cardiovascular protection and ferroptosis inhibition in other conditions. Investigating the role of ferroptosis in DCM pathogenesis, particularly in T1DM, and exploring whether irisin mitigates cardiac dysfunction through anti-ferroptotic mechanisms are crucial research aims.

Result: The study revealed lower irisin levels in the heart and serum of STZ-induced T1DM mice. Irisin supplementation improved cardiac function in diabetic cardiomyopathy (DCM) by inhibiting ferroptosis. This was shown by decreased cardiac MDA, restored GSH, and increased SLC7A11/GPX4 expression. The protective effect of irisin was demonstrated to be specific to ferroptosis, as erastin, a ferroptosis inducer, blocked irisin-mediated benefits. Mechanistically, irisin increased SIRT1 and decreased p53 K382 acetylation, leading to reduced p53 levels, upregulation of SLC7A11/GPX4, and ultimately decreasing ferroptosis and protecting cardiomyocytes from high glucose-induced injury.

Abstract | Links | Tags: M8

Cardiovascular Diabetology | 2024-04-02

Wang, Dongxu; Ren, Jing; Li, Jiping; Li, Xiuying; Ying, Jinchi; Jiang, Tiantian; Wang, Zhen; Pan, Zheng; Guo, Qianqian; Li, Chunyi; Zhang, Guokun

M8 Microscope and Scanner Utilized to Explore Deer Antler Stem Cell Conditioned Media\'s Potential in Alleviating Type 1 Diabetes via NF-κB Pathway Inhibition

A study, using the M8 microscope and slide scanner, delved into the impact of Type 1 diabetes mellitus (T1D) on human health. Type 1 diabetes mellitus (T1D) poses a significant threat to human health, primarily due to absolute insulin deficiency, leading to elevated blood glucose levels and potentially life-threatening complications such as liver injury. Mesenchymal stem cell (MSC) transplantation has emerged as a promising treatment for T1D and its associated liver injuries, leveraging the regenerative and immunomodulatory properties of MSCs. The study explored the therapeutic potential of conditioned medium from deer antler stem cells (AnSC-CM) in T1D and diabetic liver injuries. Results indicate that AnSC-CM not only alleviates T1D symptoms but also mitigates T1D-induced liver injury, outperforming bone marrow MSC-conditioned medium (BMSC-CM). Mechanistic insights suggest that the therapeutic effects of AnSC-CM may be mediated through targeting the NF-κB signaling pathway. The research proposes a novel strategy utilizing alternative stem cell conditioned mediums for effective treatment of T1D and associated liver injuries in clinical settings.

Result: Research findings demonstrated that AnSC-CM effectively relieved symptoms associated with T1D, including decreased body weight, elevated blood glucose levels, islet lesions, and diminished insulin secretion. Additionally, AnSC-CM treatment exhibited notable improvements in liver function and the mitigation of T1D-induced liver injury in mice. The therapeutic efficacy of AnSC-CM surpassed that of BMSC-CM. Analysis of underlying mechanisms unveiled significant downregulation of the NF-κB signaling pathway in both pancreatic and liver tissues by AnSC-CM. These results suggest that AnSC-CM\'s therapeutic effects on T1D and associated liver injury induced by STZ may be attributed to its modulation of the NF-κB signaling pathway.

Abstract | Links | Tags: M8

Front. Biosci. (Landmark Ed) | 2024-03-11

Hansen,; E.,; Wang,; M.,; Rolling,; C.,; & Holaska,; M., J.

PreciPoint Slide Scanning Microscope Unveils the Invasive Phenotype of MCF7 Cells under Emerin Deficiency

Using PreciPoint’s slide scanning microscope, researchers investigated how cancer cells migrate through blood vessels during metastasis. They observed that cancer cells adapt to pass through narrow gaps in the endothelium by altering their nuclei, which become more deformable. The study focused on the role of emerin protein in this process. Researchers found that invasive breast cancer cells, which have lower levels of emerin, exhibit smaller and misshapen nuclei compared to non-cancerous cells. This reduction in emerin was associated with higher rates of metastasis. To validate their hypothesis, the researchers manipulated emerin levels in noninvasive MCF7 cells and observed similar changes in nucleus size and shape, leading to impaired cell migration. This effect was consistent when emerin levels were reduced in invasive breast cancer cells. Furthermore, the analysis of breast cancer patient samples revealed a negative correlation between emerin expression and cancer invasiveness, suggesting its potential as a biomarker for tumor progression. The findings indicate that emerin loss plays a crucial role in promoting invasive transformation, highlighting its significance in cancer metastasis.

Result: To investigate how reducing emerin levels affects MCF7 cells, researchers created various MCF7 cell lines. These cell lines were engineered to produce one of three distinct emerin shRNA sequences labeled as A, B, or C, alongside a control group that expressed a scrambled shRNA sequence obtained from Genecopoeia. Immunohistochemistry and tissue microarray analyses were conducted on these cell lines. For the tissue microarray analysis, tissue microarrays underwent deparaffinization and rehydration procedures. Following additional tissue treatments, images were captured utilizing the Precipoint slide scanning microscope.

Abstract | Links | Tags: Fritz, M8

Cold Spring Harbor Laboratory | 2024-02-24

Yu, Jing; Gao, Boyuan; Li, Danning; Li, Shuang; Chiang, Vincent L.; Li, Wei; Zhou, Chenguang

M8 Microscope Reveals PtrLBD39 Overexpression Hinders Primary and Secondary Growth in Populus Trichocarpa

Researchers used M8 microscope and scanner to investigate the mechanisms underlying primary and secondary growth in trees, crucial for height and stem diameter increments, respectively, which are essential for woody biomass production. They discovered PtrLBD39, a transcription factor highly specific to stem phloem in Populus trichocarpa. Through ectopic expression of PtrLBD39 in P. trichocarpa, researchers observed a significant retardation in both primary and secondary growth. Their comprehensive analysis, including RNA-seq, ChIP-seq, and weighted gene co-expression network analysis (WGCNA), unveiled PtrLBD39\'s role in regulating transcription factors associated with vascular tissue development, wood formation, hormonal signaling, and enzymes responsible for wood components. This regulatory function resulted in growth inhibition, reduced fibrocyte secondary cell wall thickness, and diminished wood production. The study suggests that PtrLBD39 acts as a repressor, affecting both primary and secondary growth when ectopically expressed in P. trichocarpa.

Result: The stem segments, after treatment, underwent cutting into 10-micrometer sections and staining with solutions containing 5% Safranin O, 1.25% Fast Green, and 0.1% Toluidine Blue. Subsequently, micrographs of the stem sections were obtained using an M8 digital microscope and scanner. The findings of the study suggest that PtrLBD39 functions as a suppressor, impacting both primary and secondary growth upon its ectopic expression in P. trichocarpa.

Abstract | Links | Tags: M8

International Journal of Molecular Sciences | 2024-02-12

Rusche,; D.,; Englert,; N.,; Runz,; M.,; Hetjens,; S.,; Langner,; C.,; Gaiser,; T.,; & Weis,; C.-A.,

M8 Microscope and Scanner Assists Researchers in Exploring the Challenges of Detecting Tumor Budding in Colorectal Carcinoma Histology

In colorectal carcinoma (CRC) research, the M8 microscope and scanner played a pivotal role in a study aimed at predicting post-surgery treatment requirements by discerning crucial tumor features within whole slide images of solid tumors. Addressing this challenge with a small CRC dataset, researchers explored two distinct approaches. Firstly, a conventional tile-level training method was examined, incorporating various data augmentation techniques to counter the memorization effect in a noisy label setting. Secondly, a multi-instance learning (MIL) approach at the case level was explored, adapting data augmentation to prevent over-fitting in the context of limited data. The tile-level strategy proved ineffective due to a scarcity of informative image tiles per case, while the MIL approach, coupled with post-feature vector creation data augmentation, successfully predicted nodal status based on expert-derived budding scores for these cases.

Result: The dataset consisted of 29 whole slide images (WSIs), including 21 for tumors, one each for high-grade and low-grade intra-epithelial neoplasia (IEN), five for ulcerative colitis, and one for healthy tissue. The entire tissue sections were scanned and saved in the .svs format for further analysis using the M8 microscope and scanner. The study demonstrated the effectiveness of the MIL method in identifying predictive factors such as tumor budding, even within the constraints of a limited dataset size by incorporating data augmentation techniques.

Abstract | Links | Tags: M8

Applied Sciences | 2024-01-22

Stallmeyer,; B.,; Bühlmann,; C.,; Stakaitis,; R.,; Dicke,; A.-K.,; Ghieh,; F.,; Meier,; L.,; Zoch,; A.,; MacLeod,; M., D.; Steingröver,; J.,; Okutman,; Ö.,; Fietz,; D.,; Pilatz,; A.,; Escamilla,; R., A.; Xavier,; M.,; Ruckert,; C.,; Persio,; D., S.; Neuhaus,; N.,; Gurbuz,; S., A.; Şalvarci,; A.,; Tüttelmann, …; F.,

O8 Oil Microscope and Scanner Reveals Inherited Defects of piRNA Biogenesis Leading to Transposon De-Repression, Impaired Spermatogenesis, and Human Male Infertility

In an exploration of the genetic intricacies underlying piRNA dysfunction in humans, researchers leveraged the O8 oil digital microscope and slide scanner. The study delved into the critical functions of Piwi-interacting RNAs (piRNAs) in transposon silencing, germ cell maturation, and male fertility. Examining 39 infertile men with biallelic variants in 14 piRNA pathway genes, including novel candidates like PIWIL1 and GTSF1, the research uncovered distinct testicular phenotypes, ranging from complete germ cell loss to the production of abnormal spermatozoa. Notably, impaired piRNA biogenesis in spermatogonia led to transposon de-silencing, validated by elevated LINE1 expression. The study also revealed co-dependencies within the human piRNA pathway, as the abolished expression of both encoded proteins and additional piRNA factors contributed to spermatogenic failure. These findings establish the disrupted piRNA pathway as a significant cause of human infertility, providing crucial insights into transposon silencing in male germ cells.

Result: Testicular biopsies from individuals in the MERGE cohort and control subjects were acquired through testicular sperm extraction (TESE) procedures or histological assessment. These biopsies were fixed in Bouin’s solution overnight, followed by multiple processes. A the end, the processed slides were examined and documented using the O8 oil digital microscope and scanner. The study unveiled intricate co-dependencies within the human piRNA pathway. The study also demonstrated that the absence of expression in both the encoded proteins and additional piRNA factors played a significant role in contributing to spermatogenic failure.

Abstract | Links | Tags: O8

Research Square Platform LLC. | 2024-01-09

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