Scientific Publications
Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.
M8 Microscope and Scanner Used to Study the Pharmacological Impact of QM against PF in COVID -19 Patients
The coronavirus, also known as COVID-19, ravaged the world with its deadly spread in 2019. Pulmonary fibrosis (PF) occurred in severe COVID-19 patients, and signs of PF were found in the anatomical examination of the dead COVID -19 patients. On the treatment front, medical experts used Qimai Feiluoping Decoction (QM), a traditional Chinese medicine (TCM) formula, to improve the syndromes of phlegm-stasis in channels in convalescent COVID-19 patients and recommended it in a rehabilitation program for functional disorders of discharged patients. Researchers studied the pharmacological action of QM against PF from the perspective of imaging, pathological staining, and molecular mechanisms and identified possible active components.
Result: The immunohistochemistry (IHC) staining was performed to analyze the expression of PF-related biomarkers in lung tissues. After staining, the images of each section were acquired through our M8 Microscope and Scanner.
Elsevier | 2023-02-11
Fritz Slide Scanner Helps Researchers Understand the Cause of the Transformation of Murine PMCs in Connective Tissue
Researchers used our Fritz slide scanner in a study aimed at understanding the cause of the transformation of murine peritoneum-derived mast cells (PMCs) in connective tissue. Mast cells play a crucial role in both cancer progression and the immune response to infections by viruses and bacteria. They support angiogenesis, degradation of the extracellular matrix, and the transition from epithelial to mesenchymal cells. PMCs, which are mast cells isolated from the peritoneum, were the focus of the study. The goal was to determine why these PMCs were undergoing transformation.
Result: Researchers used our Fritz Slide Scanner equipped with a 40x objective and our MicroPoint software for the microscopic review and interpretation of the MGG-stained MCs. To view or transfer data, researchers used our ViewPoint and ConvertPoint software.
bioRxiv | 2023-01-24
M8 Microscope and Scanner and ViewPoint Software Unravel CARM1 and NFIB Dynamics in Small Cell Lung Cancer
Utilizing the M8 microscope and scanner, and ViewPoint software, a study unveils the pivotal role of the coactivator-associated arginine methyltransferase (CARM1) in transcriptional regulation. Researchers identified nuclear factor I B (NFIB) as a CARM1 substrate, revealing NFIB\'s dependence on CARM1 as a coactivator. Significantly, tripartite motif 29 (TRIM29) was identified as the effector molecule for methylated NFIB. Given NFIB\'s involvement in oncogenesis and metastasis, especially in small cell lung cancer (SCLC), the research explored the transformative impact of CARM1 methylation on NFIB. In an SCLC mouse model, both CARM1 and the methylation site on NFIB were found crucial for rapid SCLC onset, maintaining similar open chromatin states in tumors. These findings highlight CARM1 as a potential therapeutic target for SCLC, offering new avenues for treatment.
Result: Immunohistochemistry (IHC) was performed on formalin-fixed, paraffin-embedded mouse and human tissue sections. Sections of antibodies, CARM1 (1:1000) and NFIB (1:1000), were developed with DAB substrate and counterstained with hematoxylin. Pictures of these were taken using our M8 microscope and scanner equipped with the ViewPoint software.
Nature Communications | 2023-01-23
Our Microscope and ViewPoint in Use for Research in Fertility Feasibility in Transwomen
The study aimed to evaluate the feasibility of fertility preservation in pre-pubertal individuals undergoing gender-affirming hormone therapy. The established method of cryopreservation of immature testicular tissue, containing a population of diploid spermatogonia, is utilized as a means of preserving the spermatogenic potential of these individuals. A retrospective analysis of testicular tissue from 25 selected subjects was performed, who received a comparable hormonal regimen utilizing cyproterone acetate and estrogens.
Result: For the analysis of tissue and measurement of germ cells, the researchers used our digital microscope equipped with a 60x objective. Researchers analyzed two independent MAGE A4-stained tissue sections. The samples were then viewed using ViewPoint software, which was used to identify and count all MAGE A4-positive and -negative spermatogonia within each sample.
Reproduction and Fertility | 2023-01-18
M8 Microscope and Scanner and ViewPoint Software Unlocks PD-L1 Expression Activities in Ovarian Cancer Dynamics
Researchers investigated the potential of immune checkpoint inhibitors, specifically PD-L1 inhibitors, in treating ovarian cancer with the help of M8 microscope and scanner and ViewPoint software. Surprisingly, they found high PD-L1 expressions in 47.8% of ovarian cancer samples, spanning various epithelial ovarian cancer types. Contrary to expectations, high PD-L1 expression wasn\'t linked to poor prognosis. Additionally, 81% of ovarian cancer samples exhibited TILs expressing both CD8 and CD4, significantly correlating with elevated PD-L1 levels. Furthermore, high PD-L1 expression was associated with stem cell markers CD44 and LGR5, hinting at a connection between immune response, stem cells, and ovarian cancer. PD-L1 overexpression, especially in serous ovarian carcinomas, didn\'t impact overall survival rates. This finding underscores the intricate interplay between PD-L1, TILs, and cancer stem cells, suggesting that inhibiting PD-L1 with immune checkpoint inhibitors might curtail the stem cell population linked to cancer recurrence.
Result: Anti-CD8 (clone 4B11) mouse monoclonal antibody and Anti-CD4 (clone 4B12) mouse monoclonal antibody were used with a dilution ratio of 1:100 for tumor-infiltrating lymphocytes. The subsequent immunoreactivity was observed using M8 digital microscope and scanner. The specimen was further imaged and analyzed using ViewPoint Virtual Slide Viewing Software.
BMC Cancer | 2023-01-05
M8 Microscope and Scanner Helps Understand the Role of Connexin-43 and Ki67 in Mon-glial Central Nervous System Tumors
Intercellular communication mediated by gap junctions plays an important role in the exchange of small molecules and ions, which not only serve to maintain homeostasis but also affect cell proliferation and differentiation. The protein connexin-43 (Cx43) is involved in the formation of these gap junctional channels and hemichannels. To better understand this, researchers examined the expression of Cx43 and Ki67 in formalin-fixed, paraffin-embedded samples of human brain metastases, meningiomas and neurinomas using immunohistochemistry. To comprehensively understand the functions of connexin-43 and Ki67 within non-glial central nervous system tumors, researchers used PreciPoint’s M8 digital microscope and scanner.
Result: Researchers used our M8 Microscope and Scanner in multiple instances. M8 Microscope and Scanner 200x magnification helped researchers to understand why metastases from NSCLC did not express Cx43 as opposed to the affected cortex. Additionally, samples under M8 showed that the cellular nuclei remain unstained.
Sage Journals | 2023-01-01
Digital microscope and M8 slidescanner in use in brain research
As the control element and processing unit for information, the brain is one of the most important organs in humans. Because it is so complex, many processes in the brain are still unexplored. In their scientific report “Structural and functional connectivity from the dorsomedial hypothalamus to the ventral medulla as a chronological amplifier of sympathetic outflow”, published in the scientific journal Nature, Yosuke Kono et al. investigate the structural and functional connection of the dorsomedial hypothalamus, an area in the forebrain, and the ventral medulla, which is located in the rhomboid brain. Here, the structures of rat brains were studied as a model organism. Measurements were made by tractography, a method to visualize particle fluxes in the cells by color, and by voltage-dependent dyes, which can visualize the membrane potential in the neurons by color. Our M8 microscope was used to scan the tissue sections on which the tractography was subsequently performed.
Result: Our M8 microscope was used to scan the tissue sections on which tractography was subsequently performed.
Scientific Reports | 2022-08-07
Advancing Cancer Treatment: The M8 Microscope in Investigating Tumor-Proximate Gene Therapeutics via Clostridia
Today, medicine offers a wide variety of approaches to the treatment of cancer. Some of them are already used as standard, others are still in research, but are quite promising. In their study, Mowday et al. describe one of these research approaches in which the bacterium Clostridium sporogenes is used to effect a cure. In “Use of an optimized enzyme/prodrug combination for Clostridia directed enzyme prodrug therapy induces a significant growth delay in necrotic tumors” the bacterium is cultivated in the direct environment of the tumor tissue and is supposed to produce a therapeutic gene there. This is possible because the tumor tissue forms a nutrient environment that is selective for Clostridium sporogenes and thus promotes growth.
Result: Our digital microscope was used for the scanning tumor tissue sections that were stained with hematoxylin eosin or Gram-twort.
Cancer Gene Therapy | 2021-02-08
Decoding Skin Tumors: Proton-Sensing G-Protein Coupled Receptors and the Vital Role of the M8 Microscope
The cases of skin cancer in Germany had been rising since the last few decades and regarding a report of the health insurance company TK, every third diagnosed tumor is a skin tumor. Because of that, continuous medical research is important to understand the mechanism of tumor proliferation. This interesting topic is also investigated in the scientific paper of Klatt W., Wallner S., Brochhausen C. et al. „Expression profiles of proton‑sensing G‑protein coupled receptors in common skin tumors “. In this research, our microscope M8 was used for whole slide scanning of immunohistochemically stained tissue.
Result: Our digital scanner was not only used for generating the scans as also for analyzing the pictures with our viewer-software Viewpoint. We are proud that our product could support this research.
Scientific Reports | 2020-09-18
Researching Parkinson\'s disease using the M8 digital microscope and slide scanner
As the second most common neurodegenerative disease and 200000 deaths in 2018, Parkinson’s is one of the most insidious diseases of the 21st century. Research on the disease is ongoing throughout for better diagnoses and treatment options. Jincheol Seo et al, in their Research Article “Chronic Infiltration of T Lymphocytes into the Brain in a Non-human Primate Model of Parkinson’s Disease”of the Neuroscience of the IBR (International Brain Research Organization), describe the influence of T lymphocytes on the degeneration of neurons in the brain. As a model organism, the studies were conducted in Macaca fascicularis, Javanese monkeys.
Result: Tissue sections of the brain were immunohistochemically stained and scanned with our M8.
Neuroscience | 2020-04-01
