Scientific Publications
Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.
Digital microscope and M8 slidescanner in use for reproductive medicine research
Reproductive medicine is an important scientific topic, as it can offer help in cases of unwanted childlessness. In the study of Heckmann et al. „The initial maturation status of marmoset testicular tissues has an impact on germ cell maintenance and somatic cell response in tissue fragment culture”, our microscope helped to generate scans of testicular tissue to make certain types of cell morphologies visible.
Result: Our digital microscope was used for scanning the cell structures that were colored immunohistochemical and have a direct impact on the fertility of an individual. We are proud that our microscope is part of this paper and hope that it will also find use in some following research.
Molecular Human Reproduction | 2020-04-01
Digital microscope and slidescanner M8 in use for cancer research
Microscopy is a conventional method for experimental tests and the optical analysis of cell morphology and changes in molecular expression. As so, our M8 is used in some of these publications. Portela J., Heckmann L, Wistuba J. et al. also published a research paper with the title „Development and Disease-Dependent Dynamics of Spermatogonial Subpopulations in Human Testicular Tissues” where our M8 found a use.
Result: Our digital microscope was used for scanning testicular tissue sections that were stained histochemically and immunohistochemically. We are proud that our microscope is part of this paper and hope that it will also find use in some following research.
Journal of Clinical Medicine | 2020-01-14
Revolutionizing Leukemia Diagnosis: The Critical Role of O8 Oil Microscope in AI-Assisted Blast Cell Recognition
The reliable diagnosis of myeloid leukaemia by identifying blast cells in peripheral blood is currently still a very complicated process. The identification is carried out entirely analogously, by well-trained technical staff and doctors. On the one hand, this is precisely why the error rate is very low; on the other hand, it is not possible to define standardised procedures that would be very helpful for routine processes. In addition, the purely analogue process is very time-consuming and monotonous and therefore very tiring.
Result: One of the results of the study that we want to mention because it also plays an important role for our product O8 is the so-called saliency map. This representation visualises how important certain pixels on an image were for the analysis. In figure 1 you can see that the areas where the cells are visible in the colour image were clearly more relevant for the analysis. Thus, we can say that on the one hand the network resulted in a very specific and good recognition of the cells, but also that the scanner was able to provide high-quality images for the recognition. In addition, the good networking of our software with further interfaces for the transfer of the scanner data into the basic CNN structure offered a safe and simple further processing of the images.
Nature Machine Intelligence | 2019-11-12
Digital microscope and M8 slidescanner in use for research into neurodegenerative diseases
According to a report of The Lancet Neurology of May 2019 neurological disorders is with 9,0 million deaths and 16,5% of global deaths, the second leading cause of death after heart disease and with 276 million DALYs and 11,6% of global DALYs, it is the leading cause of disability. In the scientific paper “Abnormal Mitochondria in a Non-human Primate Model of MPTP-induced Parkinson’s Disease: Drp1 and CDK5/p25 Signaling” Park J. et al examining abnormal Mitochondria of non-human primate and the impact of it on Parkinson disease.
Result: Our digital scanner M8 was used to generate the scans of tissue sections with the immunohistochemically stained mitochondria.
Experimental Neurobiology | 2019-05-24
Digital microscope and M8 slide scanner in use Genetics research
Bin Ren et al. deal with the topic of artificial expression of bone material via adipose tissue cells in their scientific report in the scientific journal Nature, “Genes activated adipose tissue fragments as advanced autologous biomaterials for bone regeneration: osteogenic differentiation within the tissue and implications for clinical translation”. The basis for this is genetics. A specific gene, the so-called BMP-2, which is responsible for the formation of bone material, must first be integrated into the fat cells. This is done via adeno-associated viruses, which act as a smuggler of genetic material. Once in the cell nucleus, this strand is to be read with the BMP-2 and thus the desired molecule is to be produced, which later leads to bone formation. The presence of the molecule, the hBMP-2, was quantitatively checked by various optical tests.
Result: Our M8 microscope was used in this process to take scans for histomorphometry and immunohistochemical staining.
Scientific Reports | 2019-01-18
Digital microscope and M8 slidescanner used for pathology teaching
Due to the Covid-19 pandemic educations in medicine was facing new challenges, because of social distancing and new hygiene standards. With digitalization, education should overcome these challenges and because of these sudden changes, the process to come in to digital working was pushed forward very fast. Farinawati Yazid et al. published a study where they observed „The Use of Digital Microscope in Oral Pathology Teaching” by using our M8.
Result: Our digital microscope was used for scanning different slides on that the students could do the practical learning. We are proud that our microscope was part of this study. The digital transformation is a big step to overcome several challenges not only with Covid-19 but also challenges in distances coming with ongoing globalization and other problems that can be solved by digital solutions.
Journal of International Dental and Medical Research | 2019-01-05
M8 Microscope and Scanner Used in Research on ZIF-8 Sericin Hydrogel for Bone Regeneration Promoting Angiogenesis and Osteogenesis
A recent study used the M8 microscope and scanner to evaluate a novel bone regeneration scaffold. Designing scaffolds with optimal biodegradability, osteogenic potential, and angiogenic properties presents a significant challenge. Thymosin beta 10 (TMSB10), known for its roles in angiogenesis and osteogenic differentiation, faces activity preservation limitations. To address this, researchers engineered ZIF-8 as a carrier for TMSB10 (TMSB10@ZIF-8), integrating it into a self-assembled sericin hydrogel. Testing the composite in a rat cranial defect model revealed successful synthesis of TMSB10@ZIF-8 with an 88.21% encapsulation efficiency. The sustained release of TMSB10 from this composite significantly enhanced tube formation in HUVECs and promoted angiogenesis in the CAM model. Additionally, it notably improved osteogenic differentiation in MC 3 T3-E1 cells. Eight weeks post-implantation, the TMSB10@ZIF-8/Sericin hydrogel group demonstrated substantial bone healing (86.77 ± 8.91%), outperforming controls.
Result: Histological observation of the skulls was conducted using M8 microscope and scanner. The skulls were first decalcified, followed by dehydration using a graded series of ethanol. Subsequently, the samples were cleared in xylene and embedded in paraffin. Tissue slices were then obtained from the central area of each defect and subjected to staining with H&E, Masson\'s trichrome, and Sirius Red for microscopic evaluation. The approach enabled detailed examination and documentation of tissue morphology and composition within the bone defects, providing crucial insights into the efficacy of the TMSB10@ZIF-8/Sericin hydrogel scaffold in promoting bone regeneration.
International Journal of Biological Macromolecules | 0000-00-00
Research Uses ViewPoint Software to Quantify Glial Changes and Corpus Callosum Dimensions in Anorexia Nervosa Mice Models
Using the software ViewPoint, a study on anorexia nervosa (AN) revealed emaciation and neuropsychological deficits, with observed brain atrophy. This study on chronically starved mice explored glial changes in the corpus callosum (CC) and hypothalamus, critical in feeding regulation. Immunohistochemistry revealed decreased densities of OLIG2+ oligodendrocytes, GFAP+ astrocytes, and IBA1+ microglia in the CC after chronic starvation. However, glial densities in the arcuate nucleus (ARC), a key feeding center, remained unchanged. Notably, neuropeptide Y (NPY) staining intensity increased, suggesting heightened orexigenic signaling. These findings propose a role for glial pathophysiology in AN, particularly in CC alterations during chronic starvation.
Result: GFAP staining utilized sections at Bregma − 1.12 mm, whereas IBA1 and OLIG2 staining used sections at Bregma − 1.54 mm. Two immunohistochemically stained sections were digitized for each analysis, with averaged results. ViewPoint software was used to quantify GFAP+, IBA1+, and OLIG2+ cell numbers, assessed by two blinded evaluators, and presented as cells per mm2. Counting included cells with visible nuclei. Corpus callosum (CC) measurements, taken in three regions, were averaged, excluding damaged regions. This meticulous approach ensured accuracy in evaluating glial markers, cellular distribution, and CC dimensions in the designated brain sections.
Journal of Eating Disorders | 0000-00-00
