Scientific Publications
Scientific publications and exciting articles where PreciPoint products and solutions were successfully used.
M8 Microscope and Scanner and ViewPoint Software Unlocks PD-L1 Expression Activities in Ovarian Cancer Dynamics
Researchers investigated the potential of immune checkpoint inhibitors, specifically PD-L1 inhibitors, in treating ovarian cancer with the help of M8 microscope and scanner and ViewPoint software. Surprisingly, they found high PD-L1 expressions in 47.8% of ovarian cancer samples, spanning various epithelial ovarian cancer types. Contrary to expectations, high PD-L1 expression wasn\'t linked to poor prognosis. Additionally, 81% of ovarian cancer samples exhibited TILs expressing both CD8 and CD4, significantly correlating with elevated PD-L1 levels. Furthermore, high PD-L1 expression was associated with stem cell markers CD44 and LGR5, hinting at a connection between immune response, stem cells, and ovarian cancer. PD-L1 overexpression, especially in serous ovarian carcinomas, didn\'t impact overall survival rates. This finding underscores the intricate interplay between PD-L1, TILs, and cancer stem cells, suggesting that inhibiting PD-L1 with immune checkpoint inhibitors might curtail the stem cell population linked to cancer recurrence.
Result: Anti-CD8 (clone 4B11) mouse monoclonal antibody and Anti-CD4 (clone 4B12) mouse monoclonal antibody were used with a dilution ratio of 1:100 for tumor-infiltrating lymphocytes. The subsequent immunoreactivity was observed using M8 digital microscope and scanner. The specimen was further imaged and analyzed using ViewPoint Virtual Slide Viewing Software.
BMC Cancer | 2023-01-05
M8 Microscope and Scanner Helps Understand the Role of Connexin-43 and Ki67 in Mon-glial Central Nervous System Tumors
Intercellular communication mediated by gap junctions plays an important role in the exchange of small molecules and ions, which not only serve to maintain homeostasis but also affect cell proliferation and differentiation. The protein connexin-43 (Cx43) is involved in the formation of these gap junctional channels and hemichannels. To better understand this, researchers examined the expression of Cx43 and Ki67 in formalin-fixed, paraffin-embedded samples of human brain metastases, meningiomas and neurinomas using immunohistochemistry. To comprehensively understand the functions of connexin-43 and Ki67 within non-glial central nervous system tumors, researchers used PreciPoint’s M8 digital microscope and scanner.
Result: Researchers used our M8 Microscope and Scanner in multiple instances. M8 Microscope and Scanner 200x magnification helped researchers to understand why metastases from NSCLC did not express Cx43 as opposed to the affected cortex. Additionally, samples under M8 showed that the cellular nuclei remain unstained.
Sage Journals | 2023-01-01
Digital microscope and M8 slidescanner in use in brain research
As the control element and processing unit for information, the brain is one of the most important organs in humans. Because it is so complex, many processes in the brain are still unexplored. In their scientific report “Structural and functional connectivity from the dorsomedial hypothalamus to the ventral medulla as a chronological amplifier of sympathetic outflow”, published in the scientific journal Nature, Yosuke Kono et al. investigate the structural and functional connection of the dorsomedial hypothalamus, an area in the forebrain, and the ventral medulla, which is located in the rhomboid brain. Here, the structures of rat brains were studied as a model organism. Measurements were made by tractography, a method to visualize particle fluxes in the cells by color, and by voltage-dependent dyes, which can visualize the membrane potential in the neurons by color. Our M8 microscope was used to scan the tissue sections on which the tractography was subsequently performed.
Result: Our M8 microscope was used to scan the tissue sections on which tractography was subsequently performed.
Scientific Reports | 2022-08-07
Advancing Cancer Treatment: The M8 Microscope in Investigating Tumor-Proximate Gene Therapeutics via Clostridia
Today, medicine offers a wide variety of approaches to the treatment of cancer. Some of them are already used as standard, others are still in research, but are quite promising. In their study, Mowday et al. describe one of these research approaches in which the bacterium Clostridium sporogenes is used to effect a cure. In “Use of an optimized enzyme/prodrug combination for Clostridia directed enzyme prodrug therapy induces a significant growth delay in necrotic tumors” the bacterium is cultivated in the direct environment of the tumor tissue and is supposed to produce a therapeutic gene there. This is possible because the tumor tissue forms a nutrient environment that is selective for Clostridium sporogenes and thus promotes growth.
Result: Our digital microscope was used for the scanning tumor tissue sections that were stained with hematoxylin eosin or Gram-twort.
Cancer Gene Therapy | 2021-02-08
Decoding Skin Tumors: Proton-Sensing G-Protein Coupled Receptors and the Vital Role of the M8 Microscope
The cases of skin cancer in Germany had been rising since the last few decades and regarding a report of the health insurance company TK, every third diagnosed tumor is a skin tumor. Because of that, continuous medical research is important to understand the mechanism of tumor proliferation. This interesting topic is also investigated in the scientific paper of Klatt W., Wallner S., Brochhausen C. et al. „Expression profiles of proton‑sensing G‑protein coupled receptors in common skin tumors “. In this research, our microscope M8 was used for whole slide scanning of immunohistochemically stained tissue.
Result: Our digital scanner was not only used for generating the scans as also for analyzing the pictures with our viewer-software Viewpoint. We are proud that our product could support this research.
Scientific Reports | 2020-09-18
Researching Parkinson\'s disease using the M8 digital microscope and slide scanner
As the second most common neurodegenerative disease and 200000 deaths in 2018, Parkinson’s is one of the most insidious diseases of the 21st century. Research on the disease is ongoing throughout for better diagnoses and treatment options. Jincheol Seo et al, in their Research Article “Chronic Infiltration of T Lymphocytes into the Brain in a Non-human Primate Model of Parkinson’s Disease”of the Neuroscience of the IBR (International Brain Research Organization), describe the influence of T lymphocytes on the degeneration of neurons in the brain. As a model organism, the studies were conducted in Macaca fascicularis, Javanese monkeys.
Result: Tissue sections of the brain were immunohistochemically stained and scanned with our M8.
Neuroscience | 2020-04-01
Digital microscope and M8 slidescanner in use for reproductive medicine research
Reproductive medicine is an important scientific topic, as it can offer help in cases of unwanted childlessness. In the study of Heckmann et al. „The initial maturation status of marmoset testicular tissues has an impact on germ cell maintenance and somatic cell response in tissue fragment culture”, our microscope helped to generate scans of testicular tissue to make certain types of cell morphologies visible.
Result: Our digital microscope was used for scanning the cell structures that were colored immunohistochemical and have a direct impact on the fertility of an individual. We are proud that our microscope is part of this paper and hope that it will also find use in some following research.
Molecular Human Reproduction | 2020-04-01
Digital microscope and slidescanner M8 in use for cancer research
Microscopy is a conventional method for experimental tests and the optical analysis of cell morphology and changes in molecular expression. As so, our M8 is used in some of these publications. Portela J., Heckmann L, Wistuba J. et al. also published a research paper with the title „Development and Disease-Dependent Dynamics of Spermatogonial Subpopulations in Human Testicular Tissues” where our M8 found a use.
Result: Our digital microscope was used for scanning testicular tissue sections that were stained histochemically and immunohistochemically. We are proud that our microscope is part of this paper and hope that it will also find use in some following research.
Journal of Clinical Medicine | 2020-01-14
Revolutionizing Leukemia Diagnosis: The Critical Role of O8 Oil Microscope in AI-Assisted Blast Cell Recognition
The reliable diagnosis of myeloid leukaemia by identifying blast cells in peripheral blood is currently still a very complicated process. The identification is carried out entirely analogously, by well-trained technical staff and doctors. On the one hand, this is precisely why the error rate is very low; on the other hand, it is not possible to define standardised procedures that would be very helpful for routine processes. In addition, the purely analogue process is very time-consuming and monotonous and therefore very tiring.
Result: One of the results of the study that we want to mention because it also plays an important role for our product O8 is the so-called saliency map. This representation visualises how important certain pixels on an image were for the analysis. In figure 1 you can see that the areas where the cells are visible in the colour image were clearly more relevant for the analysis. Thus, we can say that on the one hand the network resulted in a very specific and good recognition of the cells, but also that the scanner was able to provide high-quality images for the recognition. In addition, the good networking of our software with further interfaces for the transfer of the scanner data into the basic CNN structure offered a safe and simple further processing of the images.
Nature Machine Intelligence | 2019-11-12
Digital microscope and M8 slidescanner in use for research into neurodegenerative diseases
According to a report of The Lancet Neurology of May 2019 neurological disorders is with 9,0 million deaths and 16,5% of global deaths, the second leading cause of death after heart disease and with 276 million DALYs and 11,6% of global DALYs, it is the leading cause of disability. In the scientific paper “Abnormal Mitochondria in a Non-human Primate Model of MPTP-induced Parkinson’s Disease: Drp1 and CDK5/p25 Signaling” Park J. et al examining abnormal Mitochondria of non-human primate and the impact of it on Parkinson disease.
Result: Our digital scanner M8 was used to generate the scans of tissue sections with the immunohistochemically stained mitochondria.
Experimental Neurobiology | 2019-05-24
