Expression profiles of proton‑sensing G‑protein coupled receptors in common skin tumors

The cases of skin cancer in Germany had been rising since the last few decades and regarding a report of the health insurance company TK, every third diagnosed tumor is a skin tumor. Because of that, continuous medical research is important to understand the mechanism of tumor proliferation. This interesting topic is also investigated in the scientific paper of Klatt W., Wallner S., Brochhausen C. et al. „Expression profiles of proton‑sensing G‑protein coupled receptors in common skin tumors “. In this research, our microscope M8 was used for whole slide scanning of immunohistochemically stained tissue.


„The proton-sensing GPCRs (pH-GPCRs) GPR4 (GPR19), TDAG8 (GPR65, T-cell death associated gene 8), OGR1 (GPR68, ovarian cancer GPCR1), and G2A (GPR132, G2 accumulation protein) are involved in sensing and transducing changes in extracellular pH (pHe). Extracellular acidification is a central hallmark of solid cancer. pH-GPCR function has been associated with cancer cell proliferation, adhesion, migration and metastasis, as well as with modulation of the immune system. Little is known about the expression levels and role of pH-GPCRs in skin cancer. To better understand the functions of pH-GPCRs in skin cancer in vivo, we examined the expression-profiles of GPR4, TDAG8, OGR1 and G2A in four common skin tumors, i.e. squamous cell carcinoma (SCC), malignant melanoma (MM), compound nevus cell nevi (NCN), basal cell carcinoma (BCC). We performed immunohistochemistry and immunofluorescence staining on paraffin-embedded tissue samples acquired from patients suffering from SCC, MM, NCN or BCC. We show the expression of pH-GPCRs in four common skin cancers. Different expression patterns in the investigated skin cancer types indicate that the different pH-GPCRs may have distinct functions in tumor progression and serve as novel therapeutic targets.”

What was the M8 used for?

Our digital scanner was not only used for generating the scans as also for analyzing the pictures with our viewer-software Viewpoint. We are proud that our product could support this research.

Discover more about the M8

Where can I find the publication?

If you want to read the whole publication, click on this link:

Expression profiles of proton-sensing G-protein coupled receptors in common skin tumors